Page 452 - IJB-10-5

International

Journal of Bioprinting

RESEARCH ARTICLE
3D-printed devices for optimized generation

of cold atmospheric plasma to improve
decontamination of surfaces from respiratory

pathogens

Asma Bouazizi 1,2† id , Klára Obrová 1† id , Eva Vaňková 3,4† id , Anna Machková 3 id ,
Josef Khun 3 id , Romana Hadravová 5 id , Jan Hodek 5 id , Lucie Ulrychová 5,6 id ,
Abdelhalim Trabelsi 2 id , Jan Weber 5 id , Leonardo Zampieri 7 id , Fabio Avino 8 id ,
Ivo Furno 8 id , Vladimír Scholtz * ,and Thomas Lion *
3 id
1,9 id
1 St. Anna Children’s Cancer Research Institute (CCRI), Vienna, Austria
2 Research Laboratory of Epidemiology and Immunogenetics of Viral Infections (LR14SP02),
Faculty of Pharmacy, University of Monastir, Tunisia
3 Department of Physics and Measurements, University of Chemistry and Technology, Prague,
Czech Republic
4 Department of Biotechnology, University of Chemistry and Technology, Prague, Czech Republic
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague,
Czech Republic
6 Department of Genetics and Microbiology, Charles University, Faculty of Sciences, Czech
Republic
† These authors contributed equally 7 Department of Physics, University of Milano Bicocca, Milano, Italy
to this work. Ecole Polytechnique Fédérale de Lausanne (EPFL), Swiss Plasma Center (SPC), Lausanne,
*Corresponding authors: Switzerland
Thomas Lion 9 Department of Pediatrics, Medical University of Vienna, Vienna, Austria
(thomas.lion@ccri.at)
Vladimír Scholz
(Vladimir.Scholtz@vscht.cz)
Citation: Bouazizi A, Obrová K, Abstract
Vaňková E, et al. 3D-printed devices
for optimized generation of cold
atmospheric plasma to improve Three-dimensional (3D)-printing technology is instrumental in creating devices for
decontamination of surfaces from biological applications, including the exploitation of cold atmospheric plasma (CAP).
respiratory pathogens. CAP, a partially ionized gas that functions at ambient temperatures, serves as a safe,
Int J Bioprint. 2024;10(5):3679.
doi: 10.36922/ijb.3679 inexpensive, and effective tool for the inactivation of various pathogens on different
surfaces. In this study, we compared three different 3D-printed devices with respect
Received: May 16, 2024 to their ability to provide optimized CAP compositions effective against select
Accepted: July 11, 2024
Published Online: August 15, 2024 respiratory viruses (SARS-CoV-2, influenza virus, adenovirus, and rhinovirus) and the
bacterium Pseudomonas aeruginosa, which is associated with serious lung diseases.
Copyright: © 2024 Author(s).
This is an Open Access article The transmission of respiratory pathogens via surface contamination may pose
distributed under the terms of the a serious health threat, thus highlighting the biological importance of the current
Creative Commons Attribution study. The properties of a prototype 3D-printed CAP-generating device and two
License, permitting distribution,
and reproduction in any medium, optimized versions were characterized by detecting reactive oxygen and nitrogen
provided the original work is species (RONS) in a gaseous environment via infrared spectroscopy and analyzing
properly cited. the composition of the reactive compounds. The virucidal effects of CAP were
Publisher’s Note: AccScience examined by determining virus infectivity and particle integrity. The bactericidal
Publishing remains neutral with effect was documented by viability testing and visualization via transmission electron
regard to jurisdictional claims in
published maps and institutional microscopy. The findings indicate that optimization of the 3D-printed devices for CAP
affiliations. production yielded an environment with relatively high amounts of RONS (O , N O,
3 2

Volume 10 Issue 5 (2024) 444 doi: 10.36922/ijb.3679