Page 55 - IJB-10-5

International

Journal of Bioprinting

REVIEW ARTICLE
In situ bioprinting: Tailored printing strategies for

regenerative medicine

Chengwei Hu 1,2 id , Chenmin Wang 1 id , Shaoquan Bian 1 id , Weichen Qi 3,4 id ,
Bo Liu 1 id , Liangliang Wang 1 id , Chunyi Wen 5 id , Jun Wu * ,
3,6 id
William W. Lu 1,3 id , and Xiaoli Zhao *
1,2 id
1 Research Center for Human Tissues and Organs Degeneration, Institute of Biomedicine and
Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy Sciences,
Shenzhen, Guangdong, China
2 University of Chinese Academy of Sciences, Beijing, China
3
Department of Orthopaedics and Traumatology, Faculty of Medicine, The University of Hong
Kong, Hong Kong, China
4 Department of Orthopedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and
Joint Diseases, Shanghai Institute of Traumatology and Orthopedics, Ruijin Hospital, Shanghai
Jiao Tong University School of Medicine, Shanghai, China
5
Department of Biomedical Engineering, Faculty of Engineering, The Hong Kong Polytechnic
University, Hong Kong, China
6 Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, Guangdong
Engineering Technology Research Center for Orthopaedic Trauma Repair, Department of
Orthopaedics and Traumatology, The University of Hong Kong-Shenzhen Hospital, Shenzhen,
Guangdong, China

Abstract
*Corresponding authors:
Xiaoli Zhao
(zhao.xl@siat.ac.cn) In recent years, three-dimensional (3D) bioprinting has emerged as a revolutionary
Jun Wu biological manufacturing technology. Despite significant progress, current
(wuj7@hku-szh.org) bioprinting technologies face critical barriers, such as the need for in vitro maturation
of printed tissues before implantation and challenges of prefabricated structures
Citation: Hu C, Wang C,
Bian S, et al. In situ bioprinting: not matching the defect shapes. In situ bioprinting has been introduced to address
Tailored printing strategies for these challenges by printing customized structures to the wound shape via direct
regenerative medicine. deposition of biological inks at the tissue interface. This paper reviews strategies
Int J Bioprint. 2024;10(5):3366.
doi: 10.36922/ijb.3366 to optimize printing performance for enhanced tissue repair and analyzes the
advantages, challenges, and future directions of in situ bioprinting technologies.
Received: April 4, 2024
Accepted: May 17, 2024
Published Online: July 24, 2024
Keywords: Bioprinting; In situ bioprinting; Tissue regeneration; Bioinks;
Copyright: © 2024 Author(s). Handheld bioprinter
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium, 1. Introduction
provided the original work is
properly cited. Over the past decade, bioprinting has gained widespread attention as a powerful
1
Publisher’s Note: AccScience tool to precisely control the spatial placement of cells and biomaterials. Bioprinting
Publishing remains neutral with refers to the bottom-up automated fabrication of scaffolds, containing living cells,
regard to jurisdictional claims in
published maps and institutional drugs, and growth factors, with the aid of additive manufacturing technology in a
affiliations. computer-aided manner. 2–4

Volume 10 Issue 5 (2024) 47 doi: 10.36922/ijb.3366