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International Journal of Bioprinting 3D-printed NAFLD model

Figure 1. Optimization of the bioink formulation for liver tissue printing. (A) 3D bioprinting system and printing model of liver tissue. (B) Printability
assessment of the bioink. (C) Biocompatibility assessment of the bioink. (D) Optimization of laminin and fibrinogen concentrations in the bioink. (E)
Effects of optimized bioink on liver function. GA Refers to Bioink Containing only GelMA and Alginate, whereas GAFL Refers to Optimized Bioink
Containing GelMA, Alginate, Laminin, and Fibrinogen.

culture environment for all cell regions. This structure culture, central necrosis occurred in the microsphere group,
facilitated uniform diffusion and transport of nutrients and indicating that the supply of oxygen and nutrients in the
oxygen, assisted in the removal of metabolic waste, and center was lacking. In contrast, the cells in the bioprinting
allowed for continuous cell proliferation, as illustrated in group survived well. The glycogen storage capacity is directly
Figure S1B and C, Supporting Information. After 7 days of related to the metabolic ability of hepatocytes. Therefore,

Volume 10 Issue 6 (2024) 365 doi: 10.36922/ijb.4312