Page 438 - IJB-10-6

International

Journal of Bioprinting

RESEARCH ARTICLE
Evaluation of 3D-bioprinted skin scaffolds in mice

along with gold nanoparticle exposure

Yi Wang 1,2 id , Xin Ma 1,3 id , Xu Wu , Shuaideng Wang , Peng Peng ,
4
1,2
1
Guozhang Tang , Xinya Qin , Xinmeng Wang * , Chenwei Wang * ,
5,6
1,3
1 id
7 id
and Jiangning Zhou 5 id
1 School of Basic Medical Sciences, Anhui Medical University, Hefei, Anhui, China
2 First School of Clinical Medicine, Anhui Medical University, Hefei, Anhui, China
3 Second School of Clinical Medicine, Anhui Medical University, Hefei, Anhui, China
4 Chinese Academy of Sciences Key Laboratory of Brain Function and Diseases, School of Life
Sciences, University of Science and Technology of China, Hefei, Anhui, China
5 Institute of Brain Science, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui,
China
6 Institute of Artificial Intelligence, Hefei Comprehensive National Science Center, Hefei, Anhui,
China
7
Department of Pharmacy, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui,
China

Abstract

The progress in nanomedicine has sparked increasing concerns regarding its
applications with biocompatible materials. Here, we assessed and optimized a three-
*Corresponding authors: dimensional (3D) bioprinting technique by testing various printing parameters
Chen-Wei Wang with multiple cell types. Cell-laden scaffolds were designed, cultivated, imaged, and
(cwwang@ustc.edu.cn) transplanted onto the dorsal skin of nude mice. The structure of bioprinted scaffolds
Xin-Meng Wang retained its shape and dimensions with no cell migration between layers. Moreover,
(wxinmeng@ustc.edu.cn)
gold nanoparticles (GNPs) were intravenously administered to transplanted nude mice
Citation: Wang Y, Ma X, Wu X, and aggregately deposited in the cell-laden scaffolds. Importantly, GNPs exhibited
et al. Evaluation of 3D-bioprinted
skin scaffolds in mice along with extensive accumulation in bioprinted scaffolds compared to natural skin and other
gold nanoparticle exposure. organs in vivo. GNPs accumulated in the dermis of the transplanted scaffolds, while
Int J Bioprint. 2024;10(6):4692. they stayed in the subcutaneous tissue of the natural skin with no permeation to the
doi: 10.36922/ijb.4692
dermis, indicating a high absorption tendency of GNPs for artificial scaffolds. The results
Received: August 29, 2024 revealed a lack of similarity between the artificial skin scaffolds and natural skin, which
Revised: September 24, 2024
Accepted: October 2, 2024 may diminish their potential as artificial skin substitutes. Furthermore, the absorption
Published Online: October 2, 2024 property of 3D-bioprinted scaffolds suggests their potential as (i) a therapeutic method
to absorb and excrete GNPs; and (ii) a strategy for targeted drug delivery of GNPs.
Copyright: © 2024 Author(s).
This is an Open Access article
distributed under the terms of the
Creative Commons Attribution Keywords: 3D bioprinting; Skin scaffolds; Gold nanoparticles; Biodistribution;
License, permitting distribution, In vivo transplant
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience
Publishing remains neutral with 1. Introduction
regard to jurisdictional claims in
published maps and institutional Within the last decade, research efforts in the field of tissue engineering continue to
1
affiliations. address the unmet need for artificial tissues and organs for transplantation. Among these

Volume 10 Issue 6 (2024) 430 doi: 10.36922/ijb.4692