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International Journal of Bioprinting Bioprinted skin scaffolds with GNP exposure

Figure 2. Construction of 3D-bioprinted scaffolds. (A) Collagen matrix and cells (RFP-HDFs, GFP-HEKs) were incorporated via layer-by-layer bioprinting
to construct the dermal and epidermal compartments. (B) Schematics of all the tested patterns of the 3D-bioprinted tissue are presented in the cross-
section view. (C–E) Before transplantation, 3D structures of three kinds of bioprinted patterns (pattern 1 [C], pattern 2 [D], and pattern 3 [E]) were
imaged and reconstructed. GFP-HEKs exhibit green fluorescence; RFP-HDFs exhibit red fluorescence. The yellow marking in Figure 2D may be unusual
aggregated cells. Scale bars: 200 μm (C–E). Abbreviations: HDFs, human dermal fibroblasts; HEKs, human epidermal keratinocytes.

Volume 10 Issue 6 (2024) 434 doi: 10.36922/ijb.4692