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International Journal of Bioprinting Bioprinted skin scaffolds with GNP exposure

Figure 6. Biodistribution of Au in collagen scaffold and mice organs. Inductively coupled plasma-optical emission spectrometry (ICP-OES) analysis of Au
concentration in the collagen scaffold and multiple organs of mice 6 days after injection with 16-nm GNPs-PEG. The results are presented as the mean ±
standard deviation. *p < 0.05; **p < 0.01; ***p < 0.005; n = 3. Abbreviations: GNP, gold nanoparticle; PEG, polyethylene glycol.

and burns require painstakingly long-term treatment, laminins, and several other glycoproteins, also contribute
drug-incorporated scaffolds have emerged as a promising to constructing a 3D macromolecular network. H&E
therapeutic strategy. 21,27 staining results indicate a lack of similarity in ECM, such
as deficiency in other acidophilic substances. We infer that
Collagen, the most abundant protein in the ECM and
known for its properties in wound healing and tissue appropriate collagen density and the addition of other
ECM components are essential to imitate natural skin.
regeneration, is one of the most widely used biopolymers
as a carrier matrix for controlled drug delivery systems. 28–30 Gold nanoparticles (GNPs) are widely used in
The biodegradable and absorbable properties of collagen, diagnostics and biosensing applications because of their
along with its non-toxic and biocompatible nature, support exquisite intrinsic properties, such as ease of synthesis
cell attachment, migration, and proliferation. The benefits and surface functionalization, tunable size and shape,
8,31
of the ECM in acute wounds and burns have also been and favorable optical, electronic, and physicochemical
demonstrated in clinical studies. In this study, the essential features. Their diagnostic applications include biosensing,
32
role of collagen in preventing cell migration between photoimaging, and bioimaging. 34–36 Additionally, GNPs
layers verified that collagen promotes the maintenance of offer slower extracellular drug release due to an adequate
cellular phenotypes. Other ECM components, such as surface-to-volume ratio, while PEG functionalization has
21
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proteoglycans/glycosaminoglycans, elastin, fibronectin, been reported to improve the stability of GNPs in complex
Volume 10 Issue 6 (2024) 439 doi: 10.36922/ijb.4692