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3D Printing of Food Foams
           into larger bubbles, which stabilize the foams. However,   XG  (Figure  1D),  and  the  difference  was  statistically
           the increase in density also makes it harder for the foams   significant.  The  spreading  was  caused  by  both  foam
           to form, resulting in a lower overrun.              instability and lack of ability to retain water. XG thickened
                                                               the foams by increasing the viscosity of the liquid phase
           (3) Foam density                                    and forming a hydrogel phase . This prevented water
                                                                                         [31]
           The EW foams (Inks 1 and 2) are generally denser than   from  spreading  on  the  filter  paper,  greatly  reducing
           the HPMC foams. The HPMC forms a light foam as a    water spreading of Inks 2, 4, and 5. Without XG, while
           result of the large overrun. The XG increases the density   Ink 1 had the protein films to hold the air bubbles and
           as shown in Figure 1C, where there is a slight increase in   water migrated to the filter paper due to strong capillary
           density from Ink 1 to Ink 2 and from Ink 3 to Ink 4. The   force. Likewise, for Ink 3, water between the air bubbles
           density affects the foaming ability inversely; as discussed   migrated from the food ink to the filter paper. Over time,
           previously, the overrun is lower when the density is   the area of wetting increased when the foam collapsed.
           higher. Ink 5 has a higher density than Ink 3 and 4 as it   For Inks 4 and 5, XG prevented the water from leaking
           contains more HPMC, thus making it more difficult to be   onto the filter paper. Over time, water evaporated, thus
           aerated and leading to a denser foam.               resulting in a slight shrinkage of the area of the foam.
           (4) Water retention                                 3.3. Rheological properties of the inks

           Syneresis refers to the undesired leakage of water from   All  the  five  inks  displayed  a  shear-thinning  or
           food. This study investigates the ability of the foam ink to   pseudoplastic property  (Figure  2A). Shear-thinning
           retain water. In the case of foams, the spreading of water   properties were preferred for extrusion printing as they
           affects  the  integrity  of  the  printed  structures,  causing   suggested  that  the  food  ink  could  be  easily  extruded
           the print to spread and collapse due to instability. In this   and  held  its  shape  after  extrusion. The  printability and
           study, the amount of water leakage from the foam inks   self-supporting structure depended on the viscosity and
                                                                                    [33]
           was obtained by measuring the area wetted on a piece of   yield stress, respectively .  The low viscosity of the
           filter paper.                                       inks allowed them to be printed easily through extrusion.
               Inks that do not have XG (Inks 1 and 3) had a large   When the shear rate increased, the viscosity of the ink
           amount of spreading compared  to the inks containing   decreased, allowing the ink to flow out smoothly. The EW


                         A                                    B















                         C                                     D
















           Figure 1. Foam properties (A). The foaming ability of each foam ink based on their overrun. (B) The foam stability of each foam ink.
           (C) The density of each foam ink. (D) The syneresis results of each ink. *P ≤ 0.1, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 for the t-test
           results (n=3).

           160                         International Journal of Bioprinting (2021)–Volume 7, Issue 4
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