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Aihemaiti, et al.
            A                                B                                C
















           D                                  E                               F



















           Figure 4. (A)Stress-strain curves of bending tests. (B-D) Flexural curve and 3DFSM images of scheme 10, scheme 8, and scheme 3.
           (E) Distorted specimens. (F) Failure of the specimen surface.


               The printing temperature  of a single deposited   was outside the sheet. The filament then began extrusion,
           line was measured and analyzed by the experimental   and when the  feeding  process was stable, the  nozzle
           setup shown in Figure 1C. The sampling frequency of   began to move toward the thin plate and began to collect
           the infrared thermal camera was set as 50 Hz, and the   deformation data. The capture frequency of the camera
           focus distance was set as approximately 70 mm from the   was set to 5 ms, and the focus distance was set to 530 mm.
           bottom surface below the stainless-steel sheet.
                                                               (8) Fracture analysis of specimens
           (7) Printing pressure measurement
                                                               To analyze the bending fracture characteristics of specimens
           The material  fusion of the FDM process relies on the   made  of  three  kinds  of  filaments,  the  cross-sectional
           temperature  of  the  extruded  molten  filament  that  heats   morphologies  of  the  three  specimens  with  different  HA
           the material in the surrounding solidified state to bond it   contents were observed using field-emission electron scanning
           together. If there is adequate pressure between the melted   microscopy (FESEM,  JSM-7610Plus, Japan).  The  three
           filament and the surrounding solidified material, it could   filaments were a PLA filament, a PLA/HA (10%) composite
           help the bonding effect between the materials.
               The stainless-steel sheet was roughed with sandpaper   filament, and a PLA/HA (20%) composite filament.
           (1800 mesh) to avoid surface reflection. Then, 75% alcohol   3. Results and discussion
           was used to clean the surface to remove the residue from
           the  stainless-steel  sheet. After  roughing,  the  sheet  was   3.1. Analysis of bending experiment results
           spray-painted, first with white paint as the background
           color and then with black as scattered spots. The sheet   Figure 4A shows the bending stress-strain curves of the
           was fixed on the platform, and the painted surface was   nine groups of specimens in the orthogonal experiment.
           faced down to the 3DFSMS. The deformation of the sheet   Table 2 shows the bending strengths of nine groups of
           was dynamically measured when a single deposited line   specimens in the orthogonal experiment.
           was printed on the upper surface of the sheet. During the   The  effects  of  the  printing  parameters  on  the
           experiment, to avoid pressure fluctuations when starting   mechanical properties were studied through the analysis of
           the filament extrusion, the initial position of the nozzle   variance (ANOVA). Table 3 shows the variance analysis
                                       International Journal of Bioprinting (2022)–Volume 8, Issue 1       157
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