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Duan, et al.
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           Figure 5. A single-level linear model of G-C, TD, and LD of age and weight. (A) The single-level linear model of G-C of age. (B) The
           single-level linear model of TD of age. (C) The single-level linear model of LD of age. (D) The single-level linear model of G-C of weight.
           (E) The single-level linear model of TD of weight. (F) The single-level linear model of LD of the weight. Abbreviations: G-C, distance from
           glottis to carina; TD, transverse diameter; LD, longitudinal diameter.

           Table 1. Multivariate analysis of sex, age, and weight of the patient as well as G-C, TD and LD
           Dependent variable  Independent variable  Coefficient  LogWorth    P      R 2   Adjusted R 2  RMSE
           G-C* (mm)                                                                 0.47     0.46       5.78
                               Sex                     1.06       1.23       0.059
                               Age (day)               0.05       6.48      0.000**
                               Weight (kg)             0.11       0.13       0.734
           TD* (mm)                                                                  0.23     0.22       0.79
                               Sex                     0.14       1.202     0.06286
                               Age (day)               0.00       1.779    0.01662*
                               Weight (kg)             0.03       0.37      0.42661
           LD* (mm)                                                                  0.19     0.17       0.62
                               Sex                     0.05       0.646     0.3772
                               Age (day)               0.00       1.117     0.0763
                               Weight (kg)             0.04       0.423     0.2257
           G-C*, distance from glottis to carina (mm); TD* (transverse diameter), to match up with the diameter of the right main bronchus (mm); LD* (longitudinal
           diameter), to match up with distance from the upper margin of the right upper lobe opening to carina (mm). *P<0.05, **P<0.01

           3.5. Verification of blocking effect of the trial-  simulating the situation of the right lung isolation during
           produced sample of BB infants only in the           thoracoscopic surgery in infants. After inflation of cuff,
           concave 3D printing model                           ink was added into the concave 3D  printed bronchial
                                                               model to observe whether there was a serious leakage
           The concave 3D printed models were used to verify   of  the  inflatable  cuff  and  whether  it  blocked  the  RUL
           the blocking validity of the trial-produced sample. The   opening at 0, 15, 30, 60, and 120 min. The experiment
           inflatable  cuff  of  BB  was  placed  between  the  opening   demonstrated  no visible  leakage at 15  min,  and slight
           of the RUL of the right main bronchus and the carina,   leakage at 30, 60, and 120 min. A silk thread through the

                                       International Journal of Bioprinting (2022)–Volume 8, Issue 3        35
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