Page 28 - IJB-8-4

Xie, et al.
A

B D

E

C F

G H I J

Figure 2. Electrospraying process analysis of TSMs. (A) Post-treatment of the received gelatin microdroplets. (B) Images captured by
high-speed camera. (C) Frequency of the droplet generation. (D) Force equilibrium of the gelatin microdroplets in the high voltage electric
field. (E) Optical images of TSMs. (F) Diameters of TSMs. (G) Viscosity stabilization duration of electrospraying ink. (H) Shear-thinning
profile of the electrospraying ink. (I) Thermo-crosslinking duration of TSMs at low temperature. (J) The stability of the crosslinked TSMs
in further bioprinting temperature.

in Figure 2B. To study the change in diameter under voltages could reach a minimum of about 800 μm. The
different electrospraying parameters, the nozzle size generation frequency would be higher when smaller
was selected as 27G, 28G, 30G and the voltages were nozzle and higher voltage applied. The flow rate of
set as 0.0 kV, 1.0 kV, 1.5 kV, 2.0 kV, 2.5 kV, 3.0 kV, and the electrospraying ink did not show obvious effect on
3.5 kV, respectively. The flow rate of electrospraying the frequency and diameter of microdroplets. From the
ink was set as 50 μL/min and 100 μL/min. The optical images shown in Figure 2E, the prepared TSMs
generation frequency of microdroplets and the diameter showed standard spheroid shape due to the existence
measurement results are shown in Figure 2C and F, of surface tension between gelatin microdroplet with
respectively. It could be found that the diameters of aqueous phase and silicon oil with oil phase. Therefore,
TSMs decreased with the reduction of the applied researchers could choose the TSMs with the required

20 International Journal of Bioprinting (2022)–Volume 8, Issue 4