Page 117 - IJB-9-1

International Journal of Bioprinting

RESEARCH ARTICLE
A 3D bioprinted tumor model fabricated with

gelatin/sodium alginate/decellularized
extracellular matrix bioink

Jie Xu , Shuangjia Yang , Ya Su , Xueyan Hu , Yue Xi , Yuen Yee Cheng ,
1†
1
2
1
1†
1
Yue Kang *, Yi Nie *, Bo Pan *, Kedong Song *
1
4,5
6
3
1 State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering,
Dalian University of Technology, Dalian 116024, China
2 Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology
Sydney, NSW 2007, Australia
3
Department of Breast Surgery, Cancer Hospital of China Medical University, 44 Xiaoheyan Road,
Dadong District, Shenyang 110042, China
4 Zhengzhou Institute of Emerging Industrial Technology, Zhengzhou 450000, China
5 Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese
Academy of Sciences, Beijing 100190, China
6 Department of Breast Surgery, The Second Hospital of Dalian Medical University, 467 Zhongshan
Road, Shahekou District, Dalian, Liaoning 116023, China
(This article belongs to the Special Issue: Advances in 3D bioprinting for regenerative medicine and
drug screening)
† These authors contributed equally Abstract
to this work.
Tissue-engineered scaffolds are more commonly used to construct three-dimension-
*Corresponding authors:
Kedong Song al (3D) tumor models for in vitro studies when compared to the conventional two-
(Kedongsong@dlut.edu.cn) dimensional (2D) cell culture because the microenvironments provided by the 3D
Yue Kang tumor models closely resemble the in vivo system and could achieve higher success
(kangyue@cancerhosp-ln-cmu.com)
Yi Nie (ynie@ipe.ac.cn); rate when the scaffolds are translated for use in pre-clinical animal model. Physical
Bo Pan (dmupanbo@hotmail.com) properties, heterogeneity, and cell behaviors of the model could be regulated to simu-
Citation: Xu J, Yang S, Su Y, et late different tumors by changing the components and concentrations of materials. In
al., 2023, A 3D bioprinted tumor this study, a novel 3D breast tumor model was fabricated by bioprinting using a bioink
model fabricated with gelatin/sodium that consists of porcine liver-derived decellularized extracellular matrix (dECM) with
alginate/decellularized extracellular
matrix bioink. Int J Bioprint, 9(1): 630. different concentrations of gelatin and sodium alginate. Primary cells were removed
https://doi.org/10.18063/ijb.v9i1.630 while extracellular matrix components of porcine liver were preserved. The rheolog-
ical properties of biomimetic bioinks and the physical properties of hybrid scaffolds
Received: May 29, 2022
Accepted: September 2, 2022 were investigated, and we found that the addition of gelatin increased hydrophilia
Published Online: October 28, and viscoelasticity, while the addition of alginate increased mechanical properties and
2022
porosity. The swelling ratio, compression modulus, and porosity could reach 835.43 ±
Copyright: © 2022 Author(s). This is 130.61%, 9.64 ± 0.41 kPa, and 76.62 ± 4.43%, respectively. L929 cells and the mouse
an Open Access article distributed breast tumor cells 4T1 were subsequently inoculated to evaluate biocompatibility of
under the terms of the Creative
Commons Attribution License, the scaffolds and to form the 3D models. The results showed that all scaffolds exhibited
permitting distribution and good biocompatibility, and the average diameter of tumor spheres could reach 148.52
reproduction in any medium, ± 8.02 μm on 7 d. These findings suggest that the 3D breast tumor model could serve
provided the original work is properly
cited. as an effective platform for anticancer drug screening and cancer research in vitro.
Publisher’s Note: Whioce
Publishing remains neutral with Keywords: Tumor model; Decellularized extracellular matrix; Gelatin; Sodium
regard to jurisdictional claims in
published maps and institutional alginate; Three-dimensional bioprinting
affiliations.

Volume 9 Issue 1 (2023) 109 https://doi.org/10.18063/ijb.v9i1.630