Page 60 - IJB-9-3

International Journal of Bioprinting

RESEARCH ARTICLE
Melt electrowriting-printed peritoneal scaffold

prevents peritoneal adhesion and facilitates
peritoneal repair

Sicheng Li , Jinjian Huang , Ziyan Xu , Ye Liu , Huajian Ren , Ze Li ,
1†
1
1
1†
2†
3
Canwen Chen , Kang Chen , Xiuwen Wu *, Jianan Ren *
1
1
1
1
1 Research Institute of General Surgery, Affiliated Jinling Hospital, Medical School of Nanjing
University, Nanjing 210002, P. R. China
2 Department of General Surgery, Nanjing First Hospital, Nanjing 210000, P. R. China
3 Research Institute of General Surgery, Jinling Hospital, School of Medicine, Southeast University,
Nanjing 210009, P. R. China
(This article belongs to the Special Issue: 3D Printing in tissue engineering)
Abstract
Peritoneal adhesion is a critical issue after abdominal surgery. Cell-based methods
for preventing peritoneal adhesion have not yet been fully investigated. Here, we
constructed a highly biomimetic peritoneal scaffold by seeding mesothelial cells, the
natural physiological barrier of the peritoneum, onto a melt electrowriting-printed
scaffold. The scaffolds with the microfibers crossed at different angles (30°, 60°, and
† These authors contributed equally
to this work. 90°) were screened based on mesothelial cell proliferation and orientation. Thirty
degrees were more suitable for improving proliferation of mesothelial cells and cell
*Corresponding authors:
Xiuwen Wu growth in a single direction; therefore, the 30° peritoneal scaffold could better mimic
(wuxiuwen@nju.edu.cn) the physiological structure of native peritoneum. Mechanistically, such a peritoneal
Jianan Ren scaffold was able to act as a barrier to prevent peritoneal resident macrophages
(jiananr@nju.edu.cn) from migrating to the site of the peritoneal lesion. In vivo mesothelial cell tracking
Citation: Li S, Huang J, Xu Z, using lentivirus technology confirmed that the peritoneal scaffold, compared to
et al., 2023, Melt electrowriting- the scaffold without mesothelial cells, could prevent peritoneal adhesion and was
printed peritoneal scaffold prevents
peritoneal adhesion and facilitates directly involved in the repair of injured peritoneum. This study suggests that the
peritoneal repair. Int J Bioprint, peritoneal scaffolds can potentially prevent peritoneal adhesion, offering a new
9(3): 682. approach for clinical treatment.
https://doi.org/10.18063/ijb.682
Received: November 13, 2022
Accepted: December 02, 2022 Keywords: Melt electrowriting; Peritoneal adhesions; Peritoneal mesothelial cells
Published Online: February 10,
2023
Copyright: © 2023 Author(s).
This is an Open Access article 1. Introduction
distributed under the terms of the
Creative Commons Attribution Peritoneal adhesion is the most common postoperative complication, which can lead to
License, permitting distribution adhesive bowel obstruction, chronic abdominal pain, and infertility [1,2] . The occurrence
and reproduction in any medium,
provided the original work is of postoperative peritoneal adhesions and their complications places a huge burden on
properly cited. patients . In 2020, a study published in the Lancet showed that during a 5-year follow-
[3]
Publisher’s Note: Whioce up period, 26.7% of patients had an average of 1.7 readmissions for adhesion-related
Publishing remains neutral with complications . Adhesive bowel obstruction is the most frequent manifestation of
[4]
regard to jurisdictional claims in peritoneal adhesion , which results in direct hospitalization costs of up to $3.45 billion
[3]
published maps and institutional
[5]
affiliations. annually in the USA .
Volume 9 Issue 3 (2023) 52 https://doi.org/10.18063/ijb.682