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International Journal of Bioprinting Peritoneal scaffolds for the peritoneal adhesion prevention

Figure 1. Structural and mechanical properties of PCL scaffolds with the fibers crossed at different angles. (A–C) The morphology of PCL scaffolds with
the fibers crossed at 90° (A), 60° (B), and 30° (C). (D) Tensile strength–strain curve of PCL scaffolds with different fiber angles. (E) Tensile strength, n = 3.
(F) Elongation at break, n = 3. (G) Young’s modulus, n = 3. (H) Fracture energy, n = 3. (I) Contact angle, n = 3. **P < 0.01; ***P < 0.001.

scattered centrifugally on the 60° scaffolds (Figure 2Ciii) peritoneal adhesion formation. To investigate the potential
and randomly on the 90° scaffolds (Figure 2Civ). mechanism of peritoneal scaffolds in the prevention of
This revealed that it was feasible to mimic the native peritoneal adhesion, we constructed a cell Transwell model
arrangement of peritoneal mesothelial cells by inducing (Figure 3A, and Figure S2), which could measure the
cells to grow orientationally rather than randomly or ability of scaffolds to hinder the recruitment and migration
centrifugally with the 30° scaffold, which is important for of adhesion-associated macrophages. The results indicated
tissue engineering . The 30° scaffold was more conducive that migration of peritoneal resident macrophages was
[31]
to the proliferation of mesothelial cells. Therefore, we significantly reduced in the peritoneal scaffold group when
chose the 30° scaffold to continue the study. compared with that in the simple PCL scaffold and blank
groups (Figure 3B and C). This suggested the advantages of
3.3. Barrier effects of the peritoneal scaffold in vitro the peritoneal scaffold acting as a physical and biological
Previous studies have shown that abdominal macrophages barrier to block adhesion of the peritoneal injury site with
play an important role in the formation of peritoneal other abdominal organs.
adhesions . Macrophages in the abdominal cavity can
[26]
rapidly move to the site of injury and generate aggregates. The selection of cell types is important when
The tissue connections bridging the aggregates and constructing a cell barrier for the prevention of peritoneal
adjacent organs are thought to be the cornerstone of adhesions. For example, Tomoya et al. proposed the
[24]

Volume 9 Issue 3 (2023) 57 https://doi.org/10.18063/ijb.682