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International Journal of Bioprinting Peritoneal scaffolds for the peritoneal adhesion prevention

Figure 3. Effects of peritoneal scaffold on blocking macrophage migration verified with a Transwell assay. (A) Transwell cell models for measurement of
scaffold’s ability to inhibit migration of macrophages. Upper chambers: 100 µL serum-free DMEM and macrophages; lower chambers: 500 µL 20% DMEM
as a chemoattractant. (i) Control group without placing PCL scaffold; (ii) placing simple PCL scaffolds; (iii) placing peritoneal scaffolds. (B) Immunoflu-
orescence staining of migrated macrophages in the lower chamber after 12 h of coculture. (C) Quantitative analysis of migrated peritoneal macrophages,
n = 3. **P < 0.01.

Figure 4. Evaluation of in vivo degradation after scaffold implantation. (A–F) HE staining confirmed the material biodegradability in mice at 1 (A), 2 (B),
4 (C), 8 (D), 12 (E), and 16 (F) weeks, postoperatively.

Volume 9 Issue 3 (2023) 59 https://doi.org/10.18063/ijb.682