Page 467 - IJB-9-5

International Journal of Bioprinting

RESEARCH ARTICLE
Silk fibroin–gelatin photo-crosslinked

3D-bioprinted hydrogel with MOF-methylene
blue nanoparticles for infected wound healing

Zhuoyuan Li , Ao Zheng 2,3† , Zhiyuan Mao , Fupeng Li , Tingshu Su ,
1
4
2,3
1†
Lingyan Cao , Wei Wang *, Yang Liu *, Chen Wang *
5
6
2,3
1
1 Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, Shanghai
Jiao Tong University School of Medicine, Shanghai, 200011, China
2 Department of Prosthodontics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University
School of Medicine; College of Stomatology, Shanghai Jiao Tong University, Shanghai, 200011,
China
3 National Center for Stomatology; National Clinical Research Center for Oral Diseases; Shanghai
Key Laboratory of Stomatology; Shanghai Engineering Research Center of Advanced Dental
Technology and Materials, Shanghai, 200011, China
4 Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai,
200011, China
5 Department of Orthopedic Surgery, Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao
† These authors contributed equally
to this work. Tong University School of Medicine, Shanghai, 200233, China
6 Department of Otolaryngology, Eye & ENT Hospital, Fudan University, Shanghai, 200031, China
*Corresponding authors:
Chen Wang
(wangchen2369@163.com)
Yang Liu
(ly1989rz@126.com) Abstract
Wei Wang
(xiaowangwei_ok@126.com) Photo-crosslinked hydrogel (PH) is an outstanding candidate for three-dimensional
(3D) printing as a wound dressing because of its high efficiency in crosslinking and
Citation: Li Z, Zheng A, Mao Z,
et al., 2023, Silk fibroin–gelatin injectability. In this study, methylene blue (MB)-loaded UiO-66(Ce) nanoparticles (NPs)
photo-crosslinked 3D-bioprinted were synthesized to prevent drug self-aggregation and achieve the photodynamic
hydrogel with MOF-methylene blue therapy (PDT) effect for efficient antibacterial action. Then, a composite photo-
nanoparticles for infected wound
healing. Int J Bioprint, 9(5): 773. crosslinked silk fibroin (SF)/gelatin hydrogel loaded with MB@UiO-66(Ce) NPs
https://doi.org/10.18063/ijb.773 (MB@UiO-66(Ce)/PH) was fabricated. The printability and the improvement of the
Received: February 10, 2023 mechanical properties of the hydrogel by the NPs were clarified. The hydrogel
Accepted: May 01, 2023 exhibited good biocompatibility and promoted the migration and proliferation of
Published Online: June 13, 2023 fibroblasts. With the PDT effect of MB@UiO-66(Ce) NPs, the hydrogel showed an
Copyright: © 2023 Author(s). excellent antibacterial effect, which became more pronounced as the concentration
This is an Open Access article increased. In vivo study showed that the MB@UiO-66(Ce)/PH could fill the defects
distributed under the terms of the
Creative Commons Attribution without gaps and accelerate the repair rate of full-thickness skin defects in mice.
License, permitting distribution, The MB@UiO-66(Ce)/PH with antibacterial properties and tissue healing-promoting
and reproduction in any medium, ability provides a new strategy involving 3D bioprinting for preparing wound
provided the original work is
properly cited. dressings.
Publisher’s Note: Whioce
Publishing remains neutral with Keywords: 3D bioprinting; Nanoparticles; Silk fibroin; Antibacterial; Photodynamic
regard to jurisdictional claims in therapy; Wound healing
published maps and institutional
affiliations.

Volume 9 Issue 5 (2023) 459 https://doi.org/10.18063/ijb.773