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International Journal of Bioprinting Vector-based G-code generation for biofabrication

infill direction and shape, as well as the sequence and slides. For example, when generating structures that
path direction. require a specific shift in orientation in the direction of the
layer infill lines—such as those mimicking collagen or cell
To illustrate this code-generation approach, several alignment in tissues like the cornea or heart—this method
exemplary bioprinted structures were created on glass proves beneficial. 35,36 As demonstrated in Figure 6A, a

Figure 6. Use of the path drawing method for generating 3D bioprinting codes. (A) A three-layer circular print with the printing path shifted by 45° for
each layer. (B) A cubical print with the spiraling blue top/bottom pattern and the red solid infill pattern. (C) An ear printed in several interconnected
layers, with unextruded connecting paths drawn in red. Embedded bioprinting of a (D) meander in the Z direction by swapping the Y for Z coordinates,
and (E) a 3D heart printed layer by layer, with each layer drawn separately. (F) The 3D cell from the institute’s logo (as shown in Figure 3G) printed with
two different materials.

Volume 11 Issue 4 (2024) 220 doi: 10.36922/ijb.6239