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Innovative Medicines & Omics                                   Impact of lemon essential oil on anthropometrics



            differences. These findings affirm the initial homogeneity   essential oil, may provide  meaningful improvements  in
            of the TG and CG, which is vital to minimize the impact of   body contour and appearance. This aligns with wellness
            confounding variables in the ensuing analyses.     objectives focused on esthetic enhancement and self-
                                                               esteem. For health outcomes, these findings suggest a
            4.2. Abdominal skinfold measurements               potential complementary role for such interventions as part
            The core focus of the study revolved around assessing   of broader lifestyle strategies targeting body composition
            the impact of lemon essential oil, the TG, on abdominal   and fat reduction.
            skinfold  measurements.  The  results  from  independent   The observed changes, while modest, underscore the
            t-tests comparing the TG and CG for skinfold thickness   feasibility of integrating affordable, non-invasive techniques
            (Table 2) offer valuable insights into this investigation.  into wellness programs. Further studies are warranted to
              Sessions 1, 2, and 4 consistently show no statistically   explore whether such interventions can produce sustained
            significant differences between the groups, regardless of   and clinically meaningful outcomes when combined with
            whether equal variances are assumed. This implies that, in   other approaches, such as diet and exercise.
            these specific sessions, lemon essential oil does not have   The  study  observed  statistically  significant
            a statistically significant effect on abdominal skinfold   improvements in abdominal skinfold thickness and other
            thickness compared to the CG.                      parameters during  these  sessions,  indicating that  the
              However, a different picture emerges in sessions 3,   intervention’s effects were not uniform across the 6 weeks.
            5, and 6. The results in these sessions reveal statistically   This variability could be attributed to differences in
            significant differences in skinfold thickness means.   individual responses  regarding physiological adaptation.
            Regarding abdominal skinfold thickness, a reduction   The body may require repeated exposure to the intervention
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            of  approximately  6.9  –  7.2  mm in  abdominal  skinfold   before showing measurable changes,  which could explain
            thickness was observed in the TG during sessions 5 and   why significant differences emerged later in the study,
            6. Abdominal skinfold measurements serve as a proxy for   such as in sessions 5 and 6. The cumulative effects of the
            subcutaneous fat levels in the abdominal region, which is   essential oil’s bioactive compounds, such as limonene,
            closely linked to overall body fat distribution.  Reducing   may amplify over time, enhancing fat mobilization or
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                                                                                                           16,18
            abdominal fat is associated with improved metabolic   improving  circulation after  multiple  applications.
            health, as excess abdominal fat is a known risk factor   Baseline variability is another potential factor. Participants’
            for conditions such as insulin resistance, cardiovascular   initial fat distribution, skin characteristics, or metabolic
            disease, and type 2 diabetes.  While the changes observed   rates could influence how quickly, and significantly, they
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            in this study are localized and relatively modest, they may   respond to the treatment.  Genetic factors affecting fat
            indicate early benefits of targeted interventions, particularly   metabolism and inflammatory responses might also
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            for individuals looking to improve body contours or reduce   contribute to the observed variations.
            fat deposits for esthetic or health reasons.         Consistency of procedures is another consideration.
              Regarding abdominal perimeter, a reduction of 3.5 cm   While the study aimed to standardize the exfoliation
            in  abdominal  perimeter  was recorded in  the  TG  during   and massage procedures, slight differences in technique
            session  5.  Waist  circumference  is  an  established  marker   (e.g., pressure, duration, or area covered) between sessions
            of central adiposity, and reductions in this measure are   or therapists could have influenced outcomes. Therapist
                                                               fatigue or variability in skill might have introduced
            linked to decreased cardiovascular and metabolic risks.    inconsistencies.  In  addition,  variations  in  participants’
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            Even small changes in waist circumference can contribute   hydration levels, food intake, or stress before measurement
            to better health outcomes, especially when sustained over   could have influenced skinfold thickness or abdominal
            time.
                                                               perimeter readings, introducing session-specific noise.
              The reductions observed in abdominal and tricep   Environmental conditions, such as room temperature
            skinfold  thickness  highlight the potential for  localized   or humidity, could have affected the penetration and
            fat loss, often a goal in body contouring and wellness   effectiveness of the essential oil.
            treatments. However, it is important to note that    Synergistic effects may also play a role. Lemon essential
            these  changes  do  not  necessarily  translate  to  systemic   oil’s bioactive compounds, such as limonene and β-pinene,
            improvements in overall fat mass or BMI, which remained   may exhibit cumulative effects when applied consistently
            unchanged.
                                                               over time.  This might explain why later sessions showed
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              The results demonstrate that non-invasive, affordable   more pronounced differences. Repeated massage sessions
            interventions, such as aromatherapy massage with lemon   could progressively improve circulation and lymphatic

            Volume 2 Issue 1 (2025)                         77                               doi: 10.36922/imo.5893
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