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Microbes & Immunity A novel anti-EphA8 monoclonal antibody
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Figure 2. Flow cytometric analysis of Ea Mab-9 in EphA8 receptor-expressed CHO-K1 and LN229 cells. (A) CHO/EphA8 (upper panels) and CHO-K1
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(lower panels) cells were treated with 0.01 – 10 μg/mL of Ea Mab-9 (red line), followed by treatment with Alexa Fluor 488-conjugated anti-mouse IgG.
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Fluorescence data were collected using the SA3800 Cell Analyzer. The black line represents the negative control (no primary antibody treatment).
(B) LN229/EphA8 (upper panels) and LN229 (lower panels) cells were treated with 0.01 – 10 μg/mL of Ea Mab-9 (red line), followed by treatment with
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Alexa Fluor 488-conjugated anti-mouse IgG. Fluorescence data were collected using the SA3800 Cell Analyzer. The black line indicates the negative
control (no primary antibody treatment).
Abbreviations: CHO: Chinese hamster ovary; EphA8: Erythropoietin-producing hepatocellular receptor A8; IgG: immunoglobulin g.
Volume 2 Issue 4 (2025) 154 doi: 10.36922/MI025060010
