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Advanced Neurology MiR-195 regulates MS-dCA1 neural circuit in CBH rat

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Figure 2. Knockdown of miR-195 reduces the dCA1-projecting neurons in MS. (A) Schematic illustration of the timeline for the injection of green
retrobeads. (B) Representative confocal images of cholinergic neurons (ChAT ) in MS region of rat administered with lenti-NC, lenti-AMO-195, and lenti-
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AMO-195 + lenti-pre-miR-195. (C) Quantitative analysis of the number of neurons double labeled by ChAT and retrobeads. (D) Quantitative analysis of
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the percentage of retrobead-labeled neuron of the ChAT-marked neurons. (E) Representative confocal images of cholinergic neurons (GAD67 ) in MS
region of rat administer with lenti-NC, lenti-AMO-195, and lenti-AMO-195 + lenti-pre-miR-195. (F) Quantitative analysis of the number of neurons
double labeled by GAD67 and retrobeads. (G) Quantitative analysis of the percentage of retrobead-labeled neuron of the GAD67 -marked neurons.
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(H) Representative confocal images of cholinergic neurons (PV ) in MS region of rat administered with lenti-NC, lenti-AMO-195, and lenti-AMO-195 +
lenti-pre-miR-195. (I) Quantitative analysis of the number of neurons double labeled by PV and retrobeads. (J) Quantitative analysis of the percentage of
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retrobead-labeled neuron of the PV -marked neurons. n = 6. *P < 0.05 versus NC rats. P < 0.05 versus lenti-AMO-195 rats.
lenti-AMO-195-injected rats than that in lenti-NC-injected process, sufficient ACh release can increase the power of
rats. Moreover, these decreased neurons were all reversed relatively low-frequency theta oscillation . Meanwhile,
[36]
by coinjection of lenti-pre-miR-195. the activation of PV GABAergic neurons in MS can elicit
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Hippocampal theta rhythmogenesis is controlled by high-frequency firing, which cooperates with cholinergic
the MS-dCA1 neural circuit depending on the synergistic neurons to maintain normal hippocampal theta rhythm .
[30]
action of cholinergic and GABAergic neurons . In this To further identify the effect of miR-195 on dCA1-projecting
[16]
Volume 1 Issue 2 (2022) 6 https://doi.org/10.36922/an.v1i2.116