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Advanced Neurology MiR-195 regulates MS-dCA1 neural circuit in CBH rat

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Figure 5. MiR-195 rescues the decreased dCA1-projecting neurons in MS of CCH rats. (A) Schematic illustration of the timeline for the injection of green
retrobeads. (B) Representative confocal images of cholinergic neurons (ChAT ) in MS region in rat from sham, 2VO, and 2VO + lenti-pre-miR-195 groups.
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(C) Quantitative analysis of the number of neurons double labeled by ChAT and retrobeads. (D) Quantitative analysis of the percentage of retrobead-
labeled neuron of the ChAT-marked neurons. (E) Representative confocal images of cholinergic neurons (GAD67 ) in MS region of rat from sham, 2VO,
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and 2VO + lenti-pre-miR-195 groups. (F) Quantitative analysis of the number of neurons double labeled by GAD67 and retrobeads. (G) Quantitative
analysis of the percentage of retrobead-labeled neuron of the GAD67 -marked neurons. (H) Representative confocal images of cholinergic neurons (PV )
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in MS region of rat from sham, 2VO, and 2VO + lenti-pre-miR-195 groups. (I) Quantitative analysis of the number of neurons double labeled by PV and
retrobeads. (J) Quantitative analysis of the percentage of retrobead-labeled neuron of the PV -marked neurons. n = 6. *P < 0.05 versus NC rats. P < 0.05
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versus lenti-AMO-195 rats.
circuit with the hippocampus that participates in the overexpressing APP, triple transgenic mice (3 × Tg), and
formation and maintenance of memory. In parallel, the wild-type mice injected with Aβ [42-44] . CCH, a preclinical
dysfunction of the MS-dCA1 neural circuit is thought to stage of MCI, has also been shown to be involved in
be one of the early pathological features of dementia [17,30,40] . impaired hippocampal theta rhythm and MS-dCA1 neural
In fact, abnormalities in neural circuit function have circuit [12,45] . Previous morphological evidence suggests
repeatedly been recorded before any neurodegeneration that the neuronal projection from the MS to the dCA1
or plaque deposition in the brain of transgenic mice pyramidal cells is mainly composed by cholinergic and

Volume 1 Issue 2 (2022) 9 https://doi.org/10.36922/an.v1i2.116