Page 90 - ARNM-3-3

Advances in Radiotherapy
& Nuclear Medicine CS@LGG for acute radiation-induced bowel injury alleviation

Appendix

A

B

C E

D F

G

Figure A1. CS@LGG displays preferable alleviation compared to standard therapies of Citamin C and GSH in radiation-induced bowel injury.
(A) Representative image of colon tissues from acute radiation-induced bowel injury animals among groups of Citamin C (500 mg/kg, PO), GSH
(300 mg/kg, IP), and CS@LGG (1 UI, PO) (n = 3). (B) Colon length statistics (n = 3). (C) Body weight changes of mice following WART (n = 3). (D) HE
staining of mouse gut tissues in different groups (scale bars: 100 μm in 200× magnification; 200 μm in 400× magnification). (E and F) Length of intestinal
villi and depth of crypts based on HE staining (n = 10). (G) Cell viability of IEC6 determined by CCK-8 assay after 8 Gy IR from day 1 to 6, treated with
Vitamin C (1 mM), GSH (1 mM), or CS@LGG (0.5 UI/mL) (n = 3). Data were analyzed by one-way ANOVA with Tukey’s post hoc test. Error bars are
presented as mean ± SD. (C) Weight changes between group IR + Vitamin C, IR + GSH, and IR + CS@LGG were calculated by an unpaired two-tailed
t-test. All experiments were independently repeated 3 times with similar results. Significant differences are indicated as follows: n.s., not significant;
*p<0.05; **p<0.01; ***p<0.001.
Abbreviations: GSH: Glutathione; HE: Hematoxylin and eosin; IP: Intraperitoneal; IR: Irradiation; OD: Optical density; PO: Per os; SD: Standard deviation;
CCK-8: Cell counting kit-8; WART: Whole-abdomen radiotherapy; LGG: Lactobacillus rhamnosus GG; CS: Chitosan.

Volume 3 Issue 3 (2025) 82 doi: 10.36922/ARNM025230026