Page 85 - ARNM-3-3

Advances in Radiotherapy
& Nuclear Medicine CS@LGG for acute radiation-induced bowel injury alleviation

A B C

D E F

G I

H

Figure 6. CS@LGG restores the gut mucus niche through B-cell immune function promotion and inflammatory pathway downregulation. (A) Venn
diagram of differential genes between three groups (n = 3). (B) PCA map of mice intestinal tract from three different treatments (n = 3). (C) Volcano plot
of differential gene expression between PBS and CS@LGG administration (n = 3). (D-F) Most enriched GO terms, including (D) biological processes,
(E) molecular function, and (F) cellular component of intestinal epithelium genes after CS@LGG treatment (n = 3). (G) KEGG pathways analysis of cellular
processes for intestinal epithelium genes (n = 3). (H) GSEA core gene clustering heatmap showing specific differential gene expression between PBS and
CS@LGG gavage (n = 3). (I) GSEA showing the key differential pathway between PBS and CS@LGG (n = 3). All experiments were independently repeated
3 times with similar results.
Abbreviations: CS: Chitosan; DEG: Differentially expressed genes; GO: Gene ontology; GSEA: Gene set enrichment analysis; KEGG: Kyoto Encyclopedia
of Genes and Genomes; LGG: Lactobacillus rhamnosus GG; PCA: Principal components analysis; PBS: Phosphate-buffered saline.

purposes. After successfully established a radiation injury in the colon and serum (Figure 2G and H). Therefore, CS@
model with severe enteritis (Figure 2A and B), we recorded LGG gavage mitigated radiation-induced damage in the
their survival time and weight gain, and analyzed samples, bowel locally and systemically.
such as blood and colon tissues. Surprisingly, we found that Furthermore, we concluded that CS@LGG can repair
CS@LGG prolonged survival time (Figure 2F), prevented the intestinal barrier microscopically. In Figure 3A, HE
weight loss (Figure 2E), reversed colon contracture staining and the related analysis of intestinal villi and crypts
(Figure 2C and D), and cleared pro-inflammatory cytokines in Figure 3B-D showed that CS@LGG administration

Volume 3 Issue 3 (2025) 77 doi: 10.36922/ARNM025230026