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Explora: Environment
            and Resource                                                   Sustainable fertilization for long bean cultivation



              Finally,  the  pod  weight per  plant was  determined  by   (week 7) to evaluate the effects of the amendments on soil
            weighing all harvested pods from each sample plant using   compaction and aeration.
            a digital balance. Harvesting was conducted 7 weeks after
            transplantation when the pods reached full maturity. Pod   3. Results and discussion
            weight, expressed in grams (g), represents the culmination   3.1. Soil characteristics and their transformation
            of the plant’s growth and reproductive processes, offering a
            comprehensive indicator of yield potential.  By analyzing   The podzolic soils used in this study were initially
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            these parameters, the study aimed to capture the full   characterized by low fertility and high acidity, conditions
            spectrum of plant responses to the integrated application of   that pose significant challenges to sustainable crop
            quail manure and natural phosphate fertilizers, providing   production. These soils exhibited a pH of 5.2, low organic
            robust evidence for the effectiveness of these sustainable   matter content (1.25%), and high aluminum saturation
            soil amendments.                                   (40%), which limits the availability of essential nutrients
                                                               such as phosphorus.  These properties are typical of
                                                                                 1
            2.7. Data analysis                                 tropical marginal soils and are often associated with poor
                                                               agricultural productivity due to their compact structure
            The collected data were analyzed using analysis of variance   and low capacity for nutrient retention. 3,24,32
            (ANOVA) to determine the significance of the main and
            interaction effects of quail manure and natural phosphate   The application of quail manure significantly improved
            fertilizers. ANOVA was chosen because it allows for the   soil characteristics by increasing organic matter content
            simultaneous comparison of multiple treatment groups   (P < 0.01), as illustrated in Figure 1, which displays notable
            and their interactions. Treatments with significant effects   enhancements in organic matter percentage (P < 0.01),
            (P < 0.05) were further analyzed using Duncan’s New   phosphorus availability (P < 0.01), and reduced aluminum
            Multiple Range Test (DNMRT) at a 5% significance level   saturation (P < 0.05). Organic matter plays a crucial role in
            to identify specific differences between treatment means.   improving soil aggregation, enhancing water infiltration,
            DNMRT was selected for its ability to control type I errors   and creating a more favorable environment for root growth.
            while providing a clear comparison of treatment effects.   In addition, as organic material decomposes, microbial
            Statistical analyses were performed using the Statistical   activity is stimulated, leading to the mineralization of
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            Package for the Social Sciences software (version 25.0; IBM   nitrogen into plant-available forms (NH₄⁺ and NO₃⁺).
            Corp., USA). 31                                    This process not only increases nitrogen availability but
                                                               also enhances soil microbial diversity, accelerating the
            2.8. Environmental and soil analysis               breakdown of organic residues and further improving soil
            Soil samples were collected before treatment application   structure. 10,13   Moreover,  the  carbon-rich  nature  of  quail
            (week 0) and after harvesting (week 7) to assess the initial   manure promotes soil aggregate stabilization, reducing
            fertility status and the effects of the amendments. For each   the risk of  erosion, improving porosity, and enhancing
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            experimental plot, three subsamples were taken at a depth   aeration.  These improvements create a more stable and
            of 0 – 20 cm and then composited into one representative   resilient soil environment, ensuring optimal conditions for
            sample per plot. This approach ensured a homogenous   plant growth by facilitating root penetration, improving
            representation of soil conditions across treatments. Soil   nutrient exchange capacity, and maintaining sufficient
            pH was measured in a 1:2.5 soil-water suspension using   oxygen supply for root respiration. The combined effects
            a pH meter (HI98128; Hanna Instruments, Romania).   of these transformations highlight the vital role of organic
            Organic matter content was determined using the    amendments  in  improving  the  fertility  and  structure  of
            Walkley-Black method, while phosphorus availability   degraded soils.
            was quantified using the Bray-1 extraction method and a   Natural phosphate fertilizer further improved soil
            spectrophotometer (Shimadzu ultraviolet-1800; Shimadzu   fertility by supplying a slow-release source of phosphorus,
            Corp., Japan). Aluminum saturation was determined   an essential  nutrient often  deficient  in  acidic  soils. The
            using the 1 M KCl extraction method, and total nitrogen   amendments reduced the chemical fixation of phosphorus
            was measured using the Kjeldahl method. These analyses   by aluminum and iron, thereby increasing its availability
            provided baseline and post-treatment soil characteristics   for plant uptake.  In addition, the combined application
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            essential for evaluating the amendments’ efficacy.  Soil   of quail manure and natural phosphate resulted in a slight
                                                     31
            bulk density was measured using the core method at   increase in soil pH, which mitigated the toxicity effects
            a depth of 0 – 10 cm and 10 – 20 cm to assess changes   of aluminum and improved overall nutrient availability.
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            in soil structure and porosity. Measurements were taken   These transformations highlight the synergistic effects
            before treatment application (week 0) and after harvesting   of  organic  and inorganic amendments  in  enhancing

            Volume 2 Issue 1 (2025)                         4                                doi: 10.36922/eer.8348
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