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Explora: Environment
and Resource Enzymatic degradation

periods lead to greater LDPE degradation, establishing a References
proportional relationship between enzyme concentration, 1. Desidery L, Lanotte M. Polymers and plastics: Types,
incubation time, and the extent of degradation. The effect of properties, and manufacturing. In: Giustozzi F,
particle size on degradation rate was determined. A higher Nizamuddin S, editors. Plastic Waste for Sustainable Asphalt
percentage degradation rate of 23.81% was observed after Roads. Woodhead Publishing Series in Civil and Structural
the 30-day incubation period for LDPE samples with a Engineering. Delhi: Woodhead Publishing; 2022. p. 3-28.
particle size of 0.5 cm. Surface imaging revealed that smaller
particle sizes exhibited rougher surfaces compared to larger doi: 10.1016/B978-0-323-85789-5.00001-0
ones, indicating more extensive surface degradation. The 2. Ho BT, Roberts TK, Lucas S. An overview on biodegradation
degradation efficiency was significantly enhanced when a of polystyrene and modified polystyrene: The microbial
two-enzyme system (Lip-Lac) was employed, compared approach. Crit Rev Biotechnol. 2018;38(2):308-20.
to single-enzyme systems. Furthermore, a decrease in doi: 10.1080/07388551.2017.1355293
particle size corresponded with an increase in the degree
of degradation. This study supports previous findings 3. Restrepo-Flórez JM, Bassi A, Thompson MR. Microbial
degradation and deterioration of polyethylene - a review. Int
that certain microbes and enzymes can be employed to Biodeterior Biodegrad. 2014;88:83-90.
degrade LDPE plastics. Future studies should explore
enzyme immobilization and reusability strategies, building doi: 10.1016/j.ibiod.2013.12.014
on the findings from this study. 4. Loredo-Treviño A, Gutiérrez-Sánchez G, Rodríguez-
Herrera R, Aguilar CN. Microbial enzymes involved in
Acknowledgments polyurethane biodegradation: A review. J Polym Environ
None. Polym Degrad. 2012;20:258-265.
doi: 10.1007/s10924-011-0390-5
Funding
5. Fujisawa M, Hirai H, Nishida T. Degradation of polyethylene
None. and nylon-66 by the laccase-mediator system. J Polym
Environ. 2001;9:103-108.
Conflict of interest
doi: 10.1023/A:1020472426516
The authors declare that they have no competing interests.
6. Anuar NFSK, Huyop F, Ur-Rehman G, et al. An overview
Author contributions into polyethylene terephthalate (PET) hydrolases and efforts
in tailoring enzymes for improved plastic degradation. Int J
Conceptualization: Muhammad Jimada Aliyu, Benjamin Mol Sci. 2022;23(20):12644.
Obinna Uloh
Formal analysis: Moses Aderemi Olutoye, Abdulhalim doi: 10.3390/ijms232012644
Musa Abubakar 7. Pires JP, Miranda GM, De Souza GL, et al. Investigation of
Investigation: Benjamin Obinna Uloh, Moses Aderemi degradation of polypropylene in soil using an enzymatic
Olutoye additive. Iran Polym J. 2019;28:1045-1055.
Methodology: Muhammad Jimada Aliyu, Abdulhalim doi: 10.1007/s13726-019-00766-8
Musa Abubakar 8. Khandare SD, Chaudhary DR, Jha B. Isolation and
Writing–original draft: Muhammad Jimada Aliyu, Moses purification of esterase enzyme from marine bacteria
Aderemi Olutoye associated with biodegradation of polyvinyl chloride (PVC).
Writing–review & editing: Abdulhalim Musa Abubakar, Biodegradation. 2025;36(4):1-20.
Benjamin Obinna Uloh
doi: 10.1007/s10532-024-10101-5
Ethics approval and consent to participate 9. Lintsen H, Hollestelle M, Hölsgens R. The Plastics
Not applicable. Revolution. How the Netherlands Became a Global Player
in Plastics. In: Gales B, Homburg E, Joosten J, Vikas
Consent for publication Sonak MA, editors. Dutch Polymer Institute (DPI), the
Netherlands Organisation for Scientific Research (NWO)
Not applicable. and Eindhoven University of Technology; 2017. p. 1-18.
Available from: https://www.polymers.nl/_asset/_public/
Availability of data news/first-instalment-the-plastics-revolution.pdf

Data are available from the corresponding author upon 10. Pilapitiya PGCNT, Ratnayake AS. The world of plastic waste:
reasonable request. A review. Clean Mater. 2024;11:100220.

Volume 2 Issue 3 (2025) 12 doi: 10.36922/EER025220042