Page 9 - EJMO-9-1

Eurasian Journal of Medicine
and Oncology

REVIEW ARTICLE
Advances and applications of single-cell

RNA sequencing in deciphering the tumor
microenvironment of malignant skin tumors

Yiting Feng 1,2† , Lanlan Liu 1,3,4,5† , Chunlan Hu 1,3,4,5† , Jie Sun 1,3,4,5 * ,
Mingzhu Yin 1,3,4,5 * , and Xin Li 1,3,4,5 *
1 Clinical Research Center, Medical Pathology Center, Cancer Early Detection and Treatment
Center, and Translational Medicine Research Center, Chongqing University Three Gorges Hospital,
Chongqing University, Chongqing, China
2 Department of Dermatology, Hunan Engineering Research Center of Skin Health and Disease,
Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University,
Changsha, Hunan, China
3 Chongqing Technical Innovation Center for Quality Evaluation and Identification of Authentic
Medicinal Herbs, Chongqing, China
4 Chongqing University Three Gorges Hospital and Academy for Advanced Interdisciplinary
Technology, CQU - Ferenc Krausz Nobel Laureate Scientific Workstation, Chongqing, China
5 School of Medicine, Chongqing University, Chongqing, China
† These authors contributed equally
*Corresponding authors:
Mingzhu Yin Abstract
(yinmingzhu@cqu.edu.cn);
Xin Li
(lixin.bioinfor@cqu.edu.cn); The three most prevalent malignant skin tumors are basal cell carcinoma (BCC),
Jie Sun squamous cell carcinoma (SCC), and cutaneous melanoma (CM). While BCC and
(sunjie@cqu.edu.cn) SCC generally exhibit low levels of malignancy, some tumors can become invasive
Citation: Feng Y, Liu L, Hu C, and metastasize. In contrast, CM is the most malignant and lethal form of skin
Sun J, Yin M, Li X. Advances cancer among these three. In recent years, the increasing incidence of skin tumors
and applications of single-cell
RNA sequencing in deciphering has garnered significant attention within the medical community. The advent of
the tumor microenvironment of single-cell RNA sequencing (scRNA-seq) technology has revolutionized the analysis
malignant skin tumors. Eurasian J of the tumor microenvironment (TME) in skin tumors, offering novel insights for
Med Oncol. 2025;9(1):1-15.
doi: 10.36922/ejmo.5809 clinical research. In this review, we provide a concise introduction to scRNA-seq
technology and its application in delineating the heterogeneity of the TME in skin
Received: November 7, 2024 tumors, elucidating the mechanisms of tumor progression and pathogenicity, and
Revised: December 2, 2024 uncovering new therapeutic targets. Our review offers a comprehensive overview for
Accepted: December 17, 2024 researchers, offering insights that may advance the understanding of the skin TME in
future studies.
Published online: January 10,
2025
Copyright: © 2025 Author(s). Keywords: Skin tumor; Tumor microenvironment; Single-cell RNA sequencing;
This is an Open-Access article Heterogeneity; Therapeutic target
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium,
provided the original work is 1. Introduction
properly cited.
Skin cancer has garnered substantial attention due to the increase in incidence, morbidity,
Publisher’s Note: AccScience
Publishing remains neutral with and mortality rates. While ultraviolet (UV) radiation is often identified as the major
regard to jurisdictional claims in risk factor for skin cancer, additional significant contributors include lifestyle choices,
published maps and institutional
1,2
affiliations workplace exposures, medical treatments, and genetic predispositions. The three most

Volume 9 Issue 1 (2025) 1 doi: 10.36922/ejmo.5809