Page 176 - EJMO-9-3

Eurasian Journal of Medicine

and Oncology

ORIGINAL RESEARCH ARTICLE
Comprehensive characterization of the

material basis and network pharmacology of
Dan-Shen-Yin in the treatment of gastritis

Jianbing Dong 1† , Zhirong Zhou 1† , Yi Chen 2† , Peng Lei 1 , Qingrui Zhang 1 ,
1,3
and Miaomiao Jiang *
1 National Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional
Chinese Medicine, Tianjin, China
2 College of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China
3 Science and Technology Project of Haihe Laboratory of Modern Chinese Medicine, Tianjin, China

Abstract

Introduction: Dan-Shen-Yin (DSY), a traditional Chinese medicinal prescription
composed of Salvia miltiorrhiza Bge. (DS), Santalum album L. (TX) and Amomum
villosum Lour. (SR), has been widely employed in the treatment of gastritis in modern
clinical practice and demonstrates excellent therapeutic efficacy. However, the main
† These authors contributed equally active ingredient and underlying mechanisms of action remain unclear.
to this work. Objective: The aim of this study is to comprehensively characterize the chemical
*Corresponding author: composition of DSY and elucidate its potential mechanisms in the treatment of gastritis.
Miaomiao Jiang Methods: The nonvolatile compounds in DSY were analyzed through liquid
(miaomiaojiang@tjutcm.edu.cn) chromatography-mass spectrometry using the data-dependent acquisition
Citation: Dong J, Zhou Z, approach, employing an in-house database. The volatile compounds and
Chen Y, et al. Comprehensive polysaccharides were detected by gas chromatography-mass spectrometry and
characterization of the material
basis and network pharmacology high-performance liquid chromatography, respectively. Network pharmacology
of Dan-Shen-Yin in the treatment analysis was performed based on the identified compounds.
of gastritis. Eurasian J Med Oncol. Results: A total of 52 nonvolatile and 15 volatile compounds were identified in
2025;9(3):168-184.
doi: 10.36922/EJMO025160124 DSY decoction, primarily including phenylpropanoids, phenols, flavonoids, and
terpenoids. Specifically, 54 compounds were found in DS, 14 in SR, and 8 in TX. DSY
Received: April 15, 2025 polysaccharide consisted of seven kinds of monosaccharides, including mannose,
Revised: May 28, 2025 rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose,
Accepted: June 20, 2025 with molar percentages of 0.21%, 0.53%, 0.15%, 4.12%, 6.24%, 18.07%, and 0.84%,
respectively. Network pharmacology analysis identified 22 key compounds among
Published online: July 17, 2025 the 67 compounds detected. These compounds exert the therapeutic effects
Copyright: © 2025 Author(s). through 55 core targets (e.g., interleukin-6, tumor necrosis factor, and TP53) and
This is an Open-Access article 10 inflammation-related pathways, such as the MAPK and nuclear factor kappa B
distributed under the terms of the
Creative Commons Attribution signaling pathways.
License, permitting distribution, Conclusion: This study further enriches the research on the material basis of DSY and
and reproduction in any medium, reveals that its volatile compounds, nonvolatile compounds, and polysaccharides
provided the original work is
properly cited. contribute to its therapeutic efficacy against gastritis. These findings may facilitate
further development and application of DSY in gastritis treatment.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: Dan-Shen-Yin; Gastritis; Chemical components; Network pharmacology
affiliations.

Volume 9 Issue 3 (2025) 168 doi: 10.36922/EJMO025160124