Page 180 - EJMO-9-3

Eurasian Journal of
Medicine and Oncology Research on DSY in treating gastritis

A

B

C

Figure 1. Optimization of GC-MS parameters for DSY decoction analysis. (A) Effect of the split ratio on peak profiles. (B) Effect of the equilibrium
temperature on peak profiles. (C) Effect of the equilibrium time on peak profiles.
Abbreviations: GC-MS: Gas chromatography-mass spectrometry; DSY: Dan-Shen-Yin.

formic acid in a water-acetonitrile system was used. In in this study, with results as shown in Figure 5. Salvinorin
addition, six kinds of chromatographic columns were A, salvinorin B, epicatechin, and orientin were used to
examined, as shown in Figure 4. The results showed screen for the optimal spray voltage in the negative ion
that Hypersil GOLD (Thermo) achieved the best peak mode, while dihydrotanshinone I, cryptotanshinone
separation and yielded the highest number of compounds– (Cry), and tanshinone IIA were used for the positive ion
totaling 1,436–as determined by SIEVE software extraction mode. Based on the peak areas of these compounds, the
in both positive and negative ion modes. Spray voltage, optimal mass spectrometry parameters were determined
capillary temperature, and auxiliary gas temperature as follows: spray voltages of 1.5 kV (positive mode) and
significantly affect the sensitivity and accuracy of mass 3 kV (negative mode), capillary temperature of 350°C, and
spectrometry. Therefore, these parameters were optimized auxiliary gas temperature of 400°C.

Volume 9 Issue 3 (2025) 172 doi: 10.36922/EJMO025160124