Page 177 - EJMO-9-3

Eurasian Journal of
Medicine and Oncology Research on DSY in treating gastritis

1. Introduction profiles of the individual herbs in DSY—especially the
nonvolatile, volatile compounds, and polysaccharides
Gastritis is an inflammatory condition of the gastric mucosa of DS—have been previously studied. 14,15 In contrast,
that can lead to symptoms such as pain, discomfort, and compositional studies of SR and TX are relatively limited,
digestive disturbances. It is typically categorized as acute, with investigations of their volatile components mainly
chronic, or specific gastritis. As a common gastrointestinal conducted by gas chromatography-mass spectrometry
1
disease, gastritis can progress to gastric cancer, one of the (GC-MS). 16,17 In addition, although DSY is commonly
most prevalent and lethal malignant tumors in the world. administered as a decoction in clinical practice, there have
2,3
Currently, clinical management of gastritis relies on been few comprehensive studies on the composition of
gastric mucosal protection and symptomatic relief, using DSY decoction.
interventions such as Vitamin C supplements, Helicobacter
pylori (H. pylori) eradication, proton pump inhibitors In this study, we aimed to comprehensively characterize
(PPIs), and non-steroidal anti-inflammatory drugs the material basis of DSY decoction using multiple analytical
(NSAIDs). However, the therapeutic effects of vitamin techniques to support foundational research on DSY.
4
C and NSAIDs in preventing the progression of gastritis Nonvolatile compounds were identified using ultra-high
to gastric cancer are unclear and are subject to ongoing performance liquid chromatography (UHPLC)–Orbitrap
debate. In addition, Du et al. conducted a systematic Exploris MS with a data-dependent acquisition method
5
4
review and meta-analysis, finding that H. pylori eradication based on an in-house database. The volatile compounds
therapy are associated with a higher incidence of adverse and polysaccharides were analyzed by headspace GC-MS
events during treatment. Moreover, the long-term use of (HS-GC-MS) and high-performance liquid chromatography
PPIs may increase the risk of gastric atrophy. Therefore, (HPLC), respectively. The relative proportions of volatile
6
further research into the prevention and management of compounds, nonvolatile compounds, and polysaccharide
gastritis is important. components in the DSY decoction were also analyzed.
Furthermore, the potential mechanisms by which these
Dan-Shen-Yin (DSY), first documented in Shi Fang Ge components exert therapeutic effects on gastritis were
Kuo (AD 1801), is a traditional Chinese herbal formula analyzed based on network pharmacology and literature-
composed of Salvia miltiorrhiza Bge. (DS), Santalum based analysis.
album L. (TX) and Amomum villosum Lour. (SR) in a
ratio of 10:1:1, prepared by water. DSY promotes blood 2. Materials and methods
circulation, removes blood stasis, promotes qi, and relieves 2.1. Reagents and chemicals
pain. It is commonly used in the treatment of gastric
and duodenal ulcers, coronary heart disease, and angina DS, SR, and TX were purchased from Beijing Tongrentang
pectoris. Clinical studies have shown that DSY exhibits Tianjin Hexi Pharmacy and their details are provided
7
significant therapeutic effects in treating gastritis. Sun in Table 1. Methanol and acetonitrile for liquid
8
et al. compared the therapeutic efficacy and safety of PPIs chromatography–mass spectrometry (LC-MS) were
9
and DSY in the treatment of gastritis, and found that DSY procured from Thermo Fisher Scientific (USA). Formic
demonstrated a significant advantage in both aspects. Wang acid was obtained from Wilmington Inc. (USA). Acetic
et al. found that DSY was effective in regulating gastric acid was purchased from Shanghai Macklin Biochemical
10
pepsinogen secretion and alleviating the severity of gastric Technology (China). Watsons deionized water (Watsons
mucosal lesions. The therapeutic effects were attributed to Corp., China) was used in all experiments.
DSY’s modulation of soluble interleukin-2 receptor, tumor A total of 28 compounds (Table S1)–purchased
necrosis factor alpha (TNF-α), prostaglandin 2 (PGE ), and either from Ichikawa Vikko Biotechnology (China) and
2
calcitonin gene-related peptide, which attenuates mucosal
inflammation and protects the gastric mucosa. Moreover, Table 1. Information on TCM prepared pieces.
pharmacological studies have confirmed that DSY enhances
Producing
Batch
ulcer healing and bolsters gastric mucosal defense. 11,12 TCM prepared pieces Latin name of number area
original plant
However, the material basis underlying DSY’s effects on
gastritis remains insufficiently understood, hindering Salviae miltiorrhizae Radix Salvia 210701 Shandong
et Rhizoma (Dan Shen, DS)
miltiorrhiza Bge.
comprehensive insight into its therapeutic mechanisms.
Amomi fructus Amomum 20210901 Guangxi
While the pharmacological actions and constituents (Sha Ren, SR) villosum Lour.
of DSY in cardiovascular diseases have been well Santali albi Lignum Santalum album 20210813 Guangdong
documented, research on its active components and (Tan Xiang, TX) L.
7,13
mechanisms in gastritis is still limited. The chemical Abbreviations: TCM: Traditional Chinese medicine
Volume 9 Issue 3 (2025) 169 doi: 10.36922/EJMO025160124