Page 181 - EJMO-9-3

Eurasian Journal of
Medicine and Oncology Research on DSY in treating gastritis

Table 2. Identification of volatile compounds in DSY decoction based on HS‑GC‑MS
No. t (min) Molecular CAS Compound name Match degree Relative Sources
R
formula number P. Match R. Match content (%)
1 4.240 C H O 2 98-00-0 2-Furanmethanol 803 830 1.065 DSY, SR, DS
5
6
2 5.444 C H O 96-48-0 Butyrolactone 860 966 0.947 DSY, SR, DS
4 6 2
3 8.787 C H O 122-78-1 Benzeneacetaldehyde 929 959 1.294 DSY, SR, DS
8 8
4 9.144 C H O 3658-77-3 Furaneol 810 836 0.410 DSY, DS
6 8 3
5 11.568 C H O 4 28564-83-2 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- 921 926 5.750 DSY, SR, DS
8
6
6 11.659 C H O 76-22-2 Camphor 811 906 2.138 DSY, TX
10
16
7 15.635 C H O 76-49-3 Bornyl acetate 834 834 0.666 DSY, SR, TX
12 20 2
8 18.588 C H O 621-59-0 Isovanillin 817 860 1.166 DSY, DS
8 8 3
9 19.016 C H O 126-86-3 2,4,7,9-Tetramethyl-5-decyn-4,7-diol 822 825 0.818 DSY, DS
14 26 2
10 21.126 C H O 96-76-4 2,4-Di-tert-butylphenol 938 941 4.684 DSY, DS
14 22
11 23.664 C H O 23811-08-7 Hinesol 854 889 0.565 DSY, DS
15
26
12 23.893 C H O 473-15-4 Beta-eudesmol 885 887 1.467 DSY, DS
26
15
13 27.793 C H O 4666-84-6 Cryptomeridiol 844 844 1.472 DSY, DS
15 28 2
14 30.888 C H O 82304-66-3 7,9-Di-tert-butyl-1- oxaspiro (4,5) deca-6,9-diene-2,8-dione 887 891 2.734 DSY, SR, DS
17 24 3
15 31.164 C H O / Diepicedrene-1-oxide 801 810 45.539 DSY, TX
15 24
Abbreviations: DS: Salvia miltiorrhiza Bge.; DSY: Dan-Shen-Yin; HS-GC-MS: Headspace gas chromatography–mass spectrometry; P.Match: Positive
match score; R.Match: Reverse match score; SR: Amomum villosum Lour.; TX: Santalum album L.; t : Retention time.
R
A B
C
D

Figure 2. HS-GC-MS TIC diagram for DSY and its individual herbs. (A) DSY. (B) DS. (C) SR. (D) TX
Abbreviations: DS: Salvia miltiorrhiza Bge.; DSY: Dan-Shen-Yin; HS-GC-MS: Headspace gas chromatography–mass spectrometry; SR: Amomum villosum
Lour.; TIC: Total ion chromatogram; TX: Santalum album L.

3.4. Identification of nonvolatile compounds in DSY In addition, as shown in Figure 6B–D, the nonvolatile
decoction compounds in the individual herbs (DS, TX, and SR) were
also identified, with details provided in Tables S6–S8. Among
A precursor ion list consisting of 304 ions (Figure 5D), the 52 compounds, 42 were derived from DS, mainly phenolic
including 163 [M+H] and 141 [M-H] , was constructed acids such as salvianolic acid A, salvianolic acid B, rosmarinic
+
-
to increase detection sensitivity for trace compounds. acid, and lithospermic acid. Eight nonvolatile compounds
Based on reference compounds and an in-house database, were derived from SR (three of which were also found in DS),
52 nonvolatile compounds in DSY were identified or mainly proanthocyanidins. Five nonvolatile compounds were
confirmed through deduction (Figure 6A), including derived from TX, mainly flavonoids. Quantitative analysis of
22 phenylpropanoids, 10 phenols, eight flavonoids, eight the original aqueous extract indicated that the proportions of
diterpenes, two oligosaccharides, a carboxylic acid, and a nonvolatile components were 70%, 73%, 66.3%, and 55.8%
benzoic acid (Table 3). for DSY, DS, TX, and SR, respectively.

Volume 9 Issue 3 (2025) 173 doi: 10.36922/EJMO025160124