Page 186 - EJMO-9-3

Eurasian Journal of
Medicine and Oncology Research on DSY in treating gastritis

method. The contents of total uronic acid and proteins in the treatment of gastritis, the polysaccharide of DSY may
DSY samples were 12.39% and 4.18%, respectively. play a significant role. Despite this, polysaccharides have
The monosaccharide composition of DSY garnered limited attention in previous gastritis studies
polysaccharide was analyzed using complete acid hydrolysis investigating DSY’s mechanisms of action.
followed by PMP derivatization and HPLC analysis. As 3.6. Network pharmacology analysis
shown in Figure 7D, seven kinds of monosaccharides
were identified, including mannose, rhamnose, glucuronic A total of 1138 targets related to 67 compounds (15 volatile
acid, galacturonic acid, glucose, galactose, and arabinose. and 52 nonvolatile) were obtained through the TCMSP,
In addition, the molar percentages of monosaccharide HERB, and SwissTargetPrediction databases. Concurrently,
composition were calculated based on their respective 1719 targets related to gastritis were obtained through the
peak areas (Table 4). The results revealed that DSY DisGeNet, GeneCards, and MalaCards databases. Venn
polysaccharide is primarily composed of galactose, diagram analysis (Figure 8A) revealed 195 overlapping
glucose, and galacturonic acid. targets, representing potential therapeutic targets of DSY for
treating gastritis. Subsequently, the compounds identified
FTIR spectroscopy (Figure 7E) confirmed the presence
of characteristic polysaccharide functional groups. in DSY decoction and these overlapping targets were
imported into Cytoscape 3.9.1 to construct a compound–
The O–H stretching vibration at 3431 cm⁻¹ indicates target–disease interaction network (Figure 8B). Network
intermolecular hydrogen bonding. The peak at 2931 cm⁻¹ topology parameters–degree, betweenness centrality,
corresponds to –CH₂– or –CH₃ stretching vibrations, and closeness centrality value–were used to evaluate
while the strong absorption at 1606 cm⁻¹ indicates the C=O
stretch of free carboxyl groups. The peaks at 1396 cm⁻¹, node importance in the network. As shown in Table 5,
926 cm⁻¹, and 871 cm⁻¹ are assigned to C–H bending, 22 compounds (5 volatile and 17 nonvolatile) exhibited
β-glycosidic, and α-glycosidic bonds, respectively. These values greater than the corresponding median values,
results are consistent with typical features of plant-derived suggesting their potential as key bioactive components of
polysaccharides. DSY in treating gastritis.
The relative contents of crude polysaccharides in their The shared targets DSY compounds and gastritis were
respective aqueous extracts were 9.8% (DSY), 12.4% (DS), imported to the STRING database, resulting in a PPI
16% (SR), and 6.4% (TX), indicating that polysaccharides network containing 194 nodes and 1142 edges (Figure 8C).
are important components of DSY, primarily derived from The CytoNCA plugin in Cytoscape was then used to assess
SR and DS. the importance of each node. Filtering conditions were
set as follows: betweenness centrality ≥median, closeness
Although polysaccharide macromolecules have centrality ≥median, degree ≥median, local average
complex structures and poor oral absorbability, studies connectivity ≥median, eigenvector ≥median, and network
have found that plant polysaccharides can exhibit ≥median. Ultimately, 55 core targets, including TNF, IL2,
biological effects in the gastrointestinal tract. DS IL6, AKT1, STAT3, and TP53, were screened from the 195
27
polysaccharides have been shown to regulate the gut intersecting targets (Figure 8C).
microbiota homeostasis. Moreover, the findings of Gao
28
et al. suggest that disruption of the gut microbiota may The GO enrichment analysis of these 55 core targets
29
be a key factor contributing to the worsening of gastric returned 1373 terms, including 1234 biological processes,
mucosal inflammation, and that restoring gut microbiota 52 cellular components, and 87 molecular functions. The
balance could be a significant approach to curing or top 10 terms in each category are shown in Figure 8D.
preventing the progression from chronic atrophic In the biological process category, the therapeutic effects
gastritis to gastric cancer. In addition, Liu et al. found of DSY appear to involve a response to reactive oxygen
30
that purified SR polysaccharide (AVLP-2) improved the species. Membrane raft, transcription regulator complex,
oxidative stress status of the gastric mucosa by increasing side of membrane, and endoplasmic reticulum lumen
superoxide dismutase activity and glutathione levels and were enriched in the cellular component category. In the
inhibiting malondialdehyde accumulation. Therefore, in molecular function category, protein kinase binding, DNA-

Table 4. Molar percentages of monosaccharide composition in DSY polysaccharide.

Monosaccharide Mannose Rhamnose Glucuronic acid Galacturonic acid Glucose Galactose Arabinose
Molar % 0.21 0.53 0.15 4.12 6.24 18.07 0.84

Volume 9 Issue 3 (2025) 178 doi: 10.36922/EJMO025160124