Page 21 - GPD-1-1

Gene & Protein in Disease

ORIGINAL RESEARCH ARTICLE
Fluorescence imaging-guided photothermal

therapy of asymmetric water-soluble
pentamethine cyanine for colorectal cancer

1†
1†
Kun Tang , Shuangshuang Jia , Yaxin Zou , Jiaheng Dong , Fangyan Liu ,
1
2
1†
Kunli Cui , Xiao Shi , and Lei Zhang *
1
3
1
1 Henan International Joint Laboratory for Nuclear Protein Regulation, School of Basic Medical
Sciences, Henan University, Kaifeng 475004, P. R. China
2 Department of Academician Shengshou Hu Workstation, Fuwai Central China Cardiovascular
Hospital, Zhengzhou 450046, P. R. China
3 Department of Pharmacy, The First Affiliated Hospital of Henan University, Henan University,
Kaifeng 475004, P. R. China
Abstract
The combination of advanced optical technology and tumor treatment has
developed into a selectively targeted tumor therapy strategy. Near-infrared (NIR)
cyanine derivative has recently received increasing attention in photothermal
therapy (PTT) because of its excellent biocompatibility, non-immunogenicity, high
fluorescence and photothermal conversion efficiency, photostability, and tumor-
homing. In this study, an asymmetric and water-soluble pentamethine cyanine was
designed and synthesized to investigate the photosensitive activity and to explore
† These authors contributed equally its potential applications as a NIR photosensitizer for the diagnosis and therapy of
to this work
colorectal cancer (CRC). The results of fluorescence imaging analysis showed that
*Corresponding author: CY5-664 preferentially accumulated in the tumor as time elapsed, and the maximum
Lei Zhang fluorescence intensity was obtained at 24 h. Cell viability and animal xenograft model
(zhlei@henu.edu.cn)
experiments showed that CY5-664 significantly inhibited tumor growth in vivo with
Citation: Tang K, Jia S, Zou Y, 0.8 W/cm of 660 nm red laser pointer for 2 min, and in vitro with 0.5 W/cm of 660 nm
2
2
et al. Fluorescence imaging-guided
photothermal therapy of asymmetric red laser pointer for 2 min. In vitro and in vivo photothermal conversion experiments
water-soluble pentamethine cyanine showed that CY5-664 exhibited an anti-CRC activity mainly through the PTT effect.
for colorectal cancer. Gene Protein Therefore, this work provides experimental evidence that CY5-664 is a drug candidate
Dis, 1(1):87.
https://doi.org/10.36922/gpd.v1i1.87 for CRC treatment mainly through the PTT effect and an alternative treatment
strategy to develop a pentamethine cyanine-based CRC theranostic photosensitizer
Received: May 8, 2022
Accepted: June 16, 2022 for synergistic CRC targeting, imaging, and therapy.
Published Online: June 29, 2022
Copyright: © 2022 Author(s). Keywords: Pentamethine cyanine; Photothermal therapy; Fluorescence imaging; Near-
This is an Open Access article infrared; Colorectal cancer
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium,
provided the original work is 1. Introduction
properly cited.
Publisher’s Note: AccScience Colorectal cancer (CRC), a common clinical malignant tumor of the digestive tract,
Publishing remains neutral with is seriously threatening human health due to the high incidence and mortality rates .
[1]
regard to jurisdictional claims in
published maps and institutional The occurrence and development of CRC are usually related to diet changes and
[2]
affiliations. environmental factors . According to the statistics on cancer in 185 countries in 2018,
Volume 1 Issue 1 (2022) 1 https://doi.org/10.36922/gpd.v1i1.87