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Gene & Protein in Disease Photothermal therapy of pentamethine cyanine

A B

C

Figure 2. Photoinduced cytotoxicity of CY5-664. (A) CY5-664 induced significant cytotoxicity under laser irradiation on HCT-116 cells. (B) CY5-664
induced significant cytotoxicity under laser irradiation on SW480 cells. (C) Morphological pictures of cells treated with different concentrations of
CY5-664 under laser irradiation on HCT-116 and SW480 cells. *P < 0.05; **P < 0.01; ***P < 0.001.
control group, there was no significant difference in body The photothermal activities of CY5-664 were evaluated
weight during the whole observation period. Liver and at three levels: Medium, intracellular, and nude mice.
kidney functions were further determined by biochemical Excitedly, the temperature of the media containing
tests of alanine aminotransferase (ALT), aspartate different concentrations of CY5-664 increased rapidly,
aminotransferase (AST), creatinine (Cre), and blood urea and the highest temperature reached 68.9°C in the media
nitrogen (BUN). As shown in Figure 3J-M, there was no containing 50 μM CY5-664 under laser irradiation
significant difference in these indicators between the CY5- (660 nm, 0.5 W/cm , 2 min) (Figure 4A). To further evaluate
2
664 + Laser irradiation group and the control group. To the effect of photothermal treatment of CY5-664 on cell
further evaluate the effects of CY5-664 on the main organs, viability, cell viability was determined by the MTT method
we carried out the organ index and H&E staining analysis with or without ice incubation for different concentrations
of the main organ sections, such as the heart, liver, spleen, of CY5-664 under laser irradiation (660 nm, 0.5 W/cm ,
2
lung, and kidney. The results showed that no significant 2 min). The results showed that ice significantly reversed
changes in organ index and abnormal histomorphology the inhibition of CY5-664 on cell viability under laser
were observed (Figure 3N and S). Overall, the above results irradiation (Figure 4B and C). Heat shock protein 70
indicated that tumor-targeted CY5-664 and imaging- (Hsp70) is a member of the most ancient and universal
guided phototherapy were highly biocompatible and low defense systems, which can be synthesized under heat
in toxic side effect. stress to achieve the purpose of cell protection . To
[37]
study the photothermal effect of CY5-664, the expression
3.4. The mechanism of CY5-664 phototherapy of HSP70 was determined using Western blotting in
Photodependent cancer therapy, including PTT and HCT116 and SW480 cell lines. The results showed that the
PDT, has emerged as a local and non-invasive therapeutic expression of HSP70 had been significantly upregulated
method for cancer therapy in recent years . For PTT, light (Figure 4D). Based on the phototherapeutic efficacy of
[35]
energy is absorbed and converted into hyperthermia by a CY5-664 in vitro, in vivo phototherapeutic activity of
photosensitizer, achieving cancer therapeutic activity . CY5-664 was initially evaluated in HCT-116 subcutaneous
[36]

Volume 1 Issue 1 (2022) 6 https://doi.org/10.36922/gpd.v1i1.87