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Gene & Protein in Disease Photothermal therapy of pentamethine cyanine

A B C D

E

F G

H I J

Figure 4. Evaluation of the photosensitive effect of CY5-664 in vitro and in vivo. (A) The temperature change of CY5-664 solution under laser irradiation
(660 nm, 0.5 W/cm , 2 min). (B) The cell viability of HCT-116 cells with or without ice incubation for different concentrations of CY5-664 under laser
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irradiation (660 nm, 0.5 W/cm , 2 min). (C) The cell viability of SW480 cells with or without ice incubation for different concentrations of CY5-664
under laser irradiation (660 nm, 0.5 W/cm , 2 min). (D–E) The HSP70 expression in HCT-116 (D) and SW480 (E) cells under laser irradiation
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(660 nm, 0.5 W/cm , 2 min); C: control, CL: control + laser. (F) The infrared ray thermal images during the 2-min laser irradiation (660 nm, 0.5 W/cm ,
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2 min). (G) The temperature curve during the 2-min laser irradiation (660 nm, 0.5 W/cm , 2 min). (H) The singlet oxygen generation of CY5-664
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solution under laser irradiation (660 nm, 0.5 W/cm , 2 min). (I) The cell viability of HCT-116 cells with or without NAC for different concentrations of
CY5-664 under laser irradiation (660 nm, 0.5 W/cm , 2 min). (J) The cell viability of SW480 cells with or without NAC for different concentrations of
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CY5-664 under laser irradiation (660 nm, 0.5 W/cm , 2 min). *P < 0.05; **P < 0.01; ***P < 0.001.
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tumor-bearing xenograft models under the guidance of property of CY5-664 in vitro and in vivo, the photodynamic
photothermal imaging. CY5-664 was injected into the activities of CY5-664 were further determined by measuring
tumor-bearing nude mice through the tail vein at 24 h the singlet oxygen production with SOSG. The results
before the laser and then the tumor was irradiated for 2 min showed that singlet oxygen production was significantly
using a 660 nm laser (0.8 W/cm ). Meanwhile, the surface induced by CY5-664 under laser irradiation (Figure 4H). To
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temperature of the tumor is monitored every 30 s using a further evaluate the intracellular photodynamic property
thermal camera. As shown in Figure 4F and G, the surface of CY5-664, cell viability was determined by the MTT
temperature of the tumor rose rapidly and reached 60°C at method with or without antioxidant N-acetyl-L-cysteine
2 min after laser irradiation in CY5-664 treatment group (NAC) for different concentrations of CY5-664 under
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mice. However, the surface temperature of the tumor only laser irradiation (660 nm, 0.5 W/cm , 2 min). However,
slightly increased to 43°C in NS groups mice. In summary, as shown in Figure 4I and J, the inhibition of HCT-116
the antitumor activities of CY5-664 were closely related to and SW480 cell viability induced by CY5-664 under laser
the strong photothermal activities. irradiation was not rescued by NAC. The cellular uptake
of CY5-664 was further determined by fluorescence
For PDT, light energy is absorbed and converted into microscope analysis at 2 h post-treatment of CY5-664. As
reactive oxygen species by a photosensitizer, achieving shown in Figure S5, CY5-664 was not uptaken by HCT116
cancer therapeutic activity [38-40] . Based on the photothermal and SW480 cells. These results showed that CY5-664 did

Volume 1 Issue 1 (2022) 8 https://doi.org/10.36922/gpd.v1i1.87