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Gene & Protein in Disease Photothermal therapy of pentamethine cyanine

A

B C

Figure 1. The synthesis scheme and spectral characteristics of CY5-664. (A) The synthesis scheme of CY5-664. (B) The spectral characteristics of CY5-664.
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(C) The photostability test of CY5-664 under laser irradiation (660 nm, 0.5 W/cm ) every 2 min.
collection committee of the Chinese Academy of Sciences CRC cell HCT-116 was harvested and suspended in
library (Shanghai, China). The cells were cultured in RPMI normal saline (NS), and was injected subcutaneously
1640 (Gibco, 11875119) medium with 10% heat-inactivated into nude mice at a concentration of 10 cells per nude
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fetal bovine serum (Gibco, 16000-044), 100 U/mL mouse. The tumor volume was estimated according to the
penicillin and 100 μg/mL streptomycin (Solarbio, P1400), following formula: L (the long diameter) × W (the short
and maintained in 5% CO at 37°C. diameter) × W × 1/2. Nude mice were randomly divided
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into three groups: (1) NS + Laser, (2) CY5-664 treated
2.3. Cell viability assay only, and (3) CY5-664 + Laser, under laser irradiation
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As previously described , HCT-116 and SW480 cells (660 nm, 0.5 W/cm ) for 2 min, until the tumor size
[24]
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were seeded in 96-well plates at a density of 5 × 10 cells reached approximately 50 mm . The CY5-664 treatment
3
per well and cultured for 12 h. CY5-664 was added into group mice were injected with CY5-664 at a concentration
culture plates according to the different predetermined of 1 mg/kg, and NS control group mice were injected with
concentrations, and the cells were cultured continuously an equal volume of NS through tail vein.
for 2 h. Cells were irradiated for 2 min (660 nm, 0.5 W/cm ),
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and the cells were cultured continuously for 24 h. The cell 2.5. In vivo NIR fluorescence imaging
viability was determined by the MTT method. In vivo NIR fluorescence imaging was observed using an
optical imaging system for small animals (IVIS lumina
2.4. In vivo experiments III, Perkinelmer, U.S.A.) at different predetermined time
In vivo experiments of this study were approved by the points after injection of CY5-664 through tail vein. Nude
Medical and Scientific Research Ethics Committee of mice were dissected and pivotal organs and tissues, such as
Henan University School of Basic Medical Sciences. heart, liver, spleen, lung, kidney, and tumor, were obtained
As previously described , the mice used in this study at 24 h after injection with CY5-664 through tail vein for
[25]
were 5-week-old female BALB/c nude mice, which were further ex vivo biodistribution assessment. The excitation
purchased from Beijing Weitonglihua Experimental wavelength is 650 nm and the emission wavelength is
Animal Technical Co., Ltd (Beijing, China). Human 670 nm in NIR fluorescence imaging analysis.

Volume 1 Issue 1 (2022) 3 https://doi.org/10.36922/gpd.v1i1.87