Page 75 - GPD-3-4

Gene & Protein in Disease

ORIGINAL RESEARCH ARTICLE
Interleukin-1β, interleukin-1Ra, and interleukin-8

in patients with SARS-CoV-2 infection: A
correlation between vaccination and clinical

outcome

Laine Andreotti Almeida 1,2 , Mikaela Nagahara 3 , Manuela dos Santos Bueno 1 ,
Mônica Pezenatto Santos 1 , Roger Labio , Spencer Luiz Marques Payão 1 ,
1
1,3
and Lucas Trevizani Rasmussen *
1 Faculdade de Medicina de Marilia (FAMEMA) - Hemocentro, Genetics Laboratory, Marília, São Paulo,
Brazil
2 Department of Veterinary Medicine, Universidade de Marília – UNIMAR, Marília, São Paulo, Brazil
3 Centro Universitário de Ourinhos – UNIFIO, Ourinhos, São Paulo, Brazil

Abstract

Coronavirus disease 2019 (COVID-19), an infectious disease caused by severe
acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was responsible for the
pandemic decreed on March 11, 2020. Here, we investigated the mRNA expression of
interleukin (IL)-1β, IL-8, and IL-1RN in samples collected from patients with and without
SARS-CoV-2 infection, who were vaccinated or unvaccinated. This investigation was
designed as a qualitative, cross-sectional, comparative, and randomized observational
study. Samples were collected through nasal/oral swabs from patients symptomatic
*Corresponding author: for influenza syndrome or SARS. Patients were categorized into three groups:
Lucas Trevizani Rasmussen DVAC (patients with SARS-CoV-2 infection and vaccinated), DNVAC (patients with
(lucasrasmussen@unifio.edu.br) SARS-CoV-2 infection and not vaccinated), and ND (patients without SARS-CoV-2
Citation: Almeida LA, Nagahara M, infection). SARS-CoV-2 was detected through reverse transcription polymerase chain
Bueno MDS, et al. Interleukin-1β, reaction (RT-PCR). Gene expressions of IL-1β, IL-8, and IL-1RN were also evaluated using
interleukin-1Ra, and interleukin-8
in patients with SARS-CoV-2 RT-PCR. Statistically significant differences in IL-8 expression were observed among
infection: A correlation between the study groups (P = 0.033); moreover, clinical signs and symptoms, frequency of
vaccination and clinical outcome. hospitalizations, and need for intensive care units showed significant differences.
Gene Protein Dis. 2024;3(4):4076.
doi: 10.36922/gpd.4076 These differences were noted in the DVAC group compared with those in the DNVAC
and ND groups. Patients with COVID-19 showed reduced IL-8 expression compared
Received: June 29, 2024 with those with other diseases that also cause influenza syndrome or SARS. These
Accepted: September 24, 2024
Published Online: November 4, results suggested that the severity of clinical manifestations of patients with COVID-19
2024 reduced after the initial two doses of the CoronaVac/Sinovac vaccine. Moreover, this
Copyright: © 2024 Author(s). vaccine reduced mortality and lethality in patients with SARS-CoV-2 infection.
This is an Open-Access article
distributed under the terms of the
Creative Commons Attribution Keywords: Severe acute respiratory syndrome coronavirus 2; Coronavirus disease 2019;
License, permitting distribution, Gene expression; Interleukins; Vaccination
and reproduction in any medium,
provided the original work is
properly cited.
Publisher’s Note: AccScience
Publishing remains neutral with 1. Introduction
regard to jurisdictional claims in
published maps and institutional Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a betacoronavirus
1
affiliations. with a positive-sense, single-stranded RNA genome. SARS-CoV-2 is responsible
Volume 3 Issue 4 (2024) 1 doi: 10.36922/gpd.4076