Page 137 - GPD-4-1

Gene & Protein in Disease

ORIGINAL RESEARCH ARTICLE
Genotypic diversity of human and porcine

group A rotaviruses in Uttar Pradesh, India

1
1
Nitin Dudhe , Kiran Bhilegaonkar * , Gazanfar Abass , Shriya Rawat 2 ,
1
1,3
Vibha Singh , Kaushal K. Rajak , Vinodh Kumar Obli Rajendran 4 ,
3
Yashpal Singh Malik , and Zunjar Baburao Dubal *
5
1
1 Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly,
Uttar Pradesh, India
2 Division of Veterinary Public Health, Sardar Vallabhbhai Patel University of Agriculture and
Technology, Meerut, Uttar Pradesh, India
3 Biological Product Division, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar
Pradesh, India
4 Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar
Pradesh, India
5 ICAR-Indian Veterinary Research Institute, Mukteswar, Uttarakhand, India

Abstract

Diarrheal diseases, particularly those caused by rotaviruses, pose a significant
health threat, especially among children, and cause huge economic losses to
*Corresponding authors: the pig industry in the form of high morbidity, mortality, and stunted growth.
Kiran Bhilegaonkar
(kiran.bhilegaonkar@icar.gov.in) Rotavirus A (RVA) remains the predominant viral agent for severe diarrheal
Zunjar Baburao Dubal episodes, contributing to high hospitalization and mortality rates in India. RVA’s
(zunjar.dubal@icar.gov.in) high genetic diversity is attributed to frequent reassortment and mutations. This
Citation: Dudhe N, Bhilegaonkar K, study aims to characterize the VP4, VP6, VP7, and NSP4 genes of RVA in stool
Abass G, et al. Genotypic diversity samples collected from children and piglets in and around Bareilly, Uttar Pradesh,
of human and porcine group A
rotaviruses in Uttar Pradesh, India. India. A total of 300 samples, including 100 from children and 200 from piglets,
Gene Protein Dis. 2025;4(1):6237. were screened for the detection of double-stranded RNA of RVA using ribonucleic
doi: 10.36922/gpd.6237 acid-polyacrylamide gel electrophoresis (RNA-PAGE) and reverse transcription
Received: November 18, 2024 polymerase chain reaction (RT-PCR). Results revealed the RVA incidence,
1st revised: December 12, 2024 particularly in winter (end of November to beginning of February), aligning with
2nd revised: December 20, 2024 observed seasonal trends. Among the 32 Rotavirus (RV)-positive samples from
3rd revised: December 24, 2024
Accepted: December 27, 2024 children, 21 (65.63%) were detected by RNA-PAGE, whereas 28 (87.5%) were
Published online: January 16, identified by RT-PCR. Whereas, of the 80 RT-PCR positive samples from piglets,
2025 only 51 (63.75%) were detected by RNA-PAGE, indicating the superiority of RT-PCR.
Copyright: © 2025 Author(s). Molecular analysis identified the prevalent genotypes in human strains as G1, G2,
This is an Open-Access article G3, and P[8], whereas G9P[13]-I5-E1 dominated among piglets in a single farm
distributed under the terms of the
Creative Commons Attribution outbreak. The findings underscore the critical need for continuous surveillance
License, permitting distribution, to monitor evolving RV genotypes from both humans and piglets, enabling the
and reproduction in any medium, identification of new strains of RVA and subsequent modification of vaccination
provided the original work is
properly cited. strategies to reduce RVA’s impact in India.
Publisher’s Note: AccScience
Publishing remains neutral with Keywords: Diarrheal diseases; Epidemiology; Genotypic diversity; India; RNA-PAGE;
regard to jurisdictional claims in
published maps and institutional Rotavirus A; Reverse transcription polymerase chain reaction
affiliations.

Volume 4 Issue 1 (2025) 1 doi: 10.36922/gpd.6237