Page 41 - GTM-1-1

Global Translational Medicine

ORIGINAL RESEARCH ARTICLE
Identification of potential hub genes

for the diagnosis and therapy of dilated
cardiomyopathy with heart failure through

bioinformatics analysis

5
2,4
5
Xinghui Zhuang 1,2† , Mengyue Tian , Liming Li , Shurong Xu , Meiling Cai ,
3†
Xiaojie Yang , Zhihuang Qiu , Tianci Chai 1,2,6 , and Liangwan Chen *
2,4
1,2
1,2
1 Department of Cardiovascular Surgery, Fujian Medical University Union Hospital, Fuzhou, China
2 Key Laboratory of Cardio-Thoracic Surgery (Fujian Medical University), Fujian Province University,
Fuzhou, China
3 Key Laboratory of Ministry of Education for Gastrointestinal Caner, School of Basic Medical
Sciences, Fujian Medical University, Fuzhou, China
4 Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, China
5 Department of Nursing, Fujian Medical University Union Hospital, Fuzhou, China
6 Department of Anesthesiology, Xinyi People’s Hospital, Xuzhou, China

Abstract

Dilated cardiomyopathy (DCM) is a common cause of heart failure. However, genetic-
level treatments are not available for this condition. In this study, we searched for
† These authors contributed equally
to this work biological markers and therapeutic targets for DCM from a genetic perspective. We
*Corresponding author: chose microarray datasets of idiopathic DCM with heart failure tissues and normal
Liangwan Chen function (NF) heart tissues, which were downloaded from the Gene Expression
(chenliangwan@fjmu.edu.cn)
Omnibus (GEO) database. The differentially expressed genes (DEGs) were analyzed
Citation: Zhuang X, Tian M, Li L, by the GEO2R tool. Gene ontology (GO) and gene set enrichment analysis were used
et al. 2022, Identification of potential
hub genes for the diagnosis and to analyze the functions of DEGs and the pathways in which they are involved. Next,
therapy of dilated cardiomyopathy protein-protein interaction networks were built to filter out the hub genes from DEGs.
with heart failure through The expression of hub gene was validated by other GEO datasets. Receiver operating
bioinformatics analysis. Global
Transl Med, 1(1): 104. characteristic (ROC) curves were plotted to verify the accuracy of the genetic diagnosis.
https://doi.org/10.36922/gtm.v1i1.104 In the end, the mRNA-miRNA-lncRNA network was built to find potentially correlative
Received: May 20, 2022 genes. Twenty-eight common DEGs in total were screened, and GO analysis showed
Accepted: June 16, 2022 that DEGs were mainly associated with neutrophil degranulation and activation,
Published Online: June 28, 2022 regulation of Wnt signaling pathway and the development of cardiac cell and tissue.
Copyright: © 2022 Author(s). Five hub genes (asporin [ASPN], osteoglycin [OGN], secreted frizzled-related protein 4
This is an Open Access article [SFRP4], membrane metalloendopeptidase [MME], and natriuretic peptide gene [NPPA])
distributed under the terms of the were shown to be highly expressed in the validation sets and accurate in distinguish
Creative Commons Attribution
License, permitting distribution, between DCM and NF by ROC curves. miRNA prediction of the hub genes revealed that
and reproduction in any medium, hsa-mir-28b-5p was associated with SFRP4, ASPN, and MME. All of them may serve as
provided the original work is biological diagnostic indicators and provide direction for treatment at the genetic level.
properly cited.
Publisher’s Note: AccScience
Publishing remains neutral with Keywords: Dilated cardiomyopathy; Heart failure; Bioinformatics analysis; Differentially
regard to jurisdictional claims in expressed genes; Hub genes
published maps and institutional
affiliations.

Volume 1 Issue 1 (2022) 1 https://doi.org/10.36922/gtm.v1i1.104