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Global Translational Medicine Bioinformatics analysis of dilated cardiomyopathy

Table 1. Remarkably enriched biological processes of the Table 1. (Continued).
differentially expressed genes.
ID Term Gene P value
ID Term Gene P value counts
counts GO:0055007 Cardiac muscle cell 3 9.94e-04
GO:0043312 Neutrophil degranulation 6 6.90e-05 differentiation
GO:0002283 Neutrophil activation involved 6 7.14e-05 GO:0033006 Regulation of mast cell 2 0.001
in immune response activation involved in immune
response
GO:0050729 Positive regulation of 4 7.62e-05
inflammatory response GO:0035567 Non-canonical Wnt signaling 3 0.001
pathway
GO:0042119 Neutrophil activation 6 7.99e-05
GO:0060070 Canonical Wnt signaling 4 0.001
GO:0002446 Neutrophil mediated immunity 6 8.08e-05
pathway
GO:0032103 Positive regulation of response 5 1.06e-04
to external stimulus GO:0030049 Muscle filament sliding 2 0.002
GO:0033275 Actin-myosin filament sliding 2 0.002
GO:0008217 Regulation of blood pressure 4 1.49e-04
GO:0031589 Cell-substrate adhesion 5 1.64e-04
GO:0033008 Positive regulation of mast cell 2 2.25e-04 Table 2. Remarkably enriched cellular components of the
activation involved in immune differentially expressed genes.
response
ID Term Gene P value
GO:0043306 Positive regulation of mast cell 2 2.25e-04 counts
degranulation GO:0062023 Collagen-containing 11 3.87e-12
GO:0060026 Convergent extension 2 2.25e-04 extracellular matrix
GO:0055013 Cardiac muscle cell development 3 3.15e-04 GO:0005766 Primary lysosome 3 0.001
GO:0007160 Cell-matrix adhesion 4 3.35e-04 GO:0042582 Azurophil granule 3 0.001
GO:0043062 Extracellular structure 5 3.69e-04 GO:0005775 Vacuolar lumen 3 0.002
organization
GO:0055006 Cardiac cell development 3 3.81e-04
software, and the results are shown in Figure 6. The deeper
GO:0048738 Cardiac muscle tissue 4 3.82e-04
development the color is, the more points it scored. Asporin (ASPN),
GO:0033005 Positive regulation of mast cell 2 4.05e-04 secreted frizzled-related protein 4 (SFRP4), membrane
activation metalloendopeptidase (MME), natriuretic peptide A gene
GO:0048705 Skeletal system morphogenesis 4 4.21e-04 (NPPA), and osteoglycin (OGN) were found to be the hub
GO:2000050 Regulation of non-canonical 2 5.38e-04 genes, and they were all highly expressed in DCM with
Wnt signaling pathway heart failure.
GO:0043302 Positive regulation of leukocyte 2 5.87e-04 3.4. Validation of hub gene expression
degranulation
GO:0060349 Bone morphogenesis 3 6.50e-04 GSE5406 and GSE79962 were used as the validation sets
GO:0033688 Regulation of osteoblast 2 8.00e-04 in the present study. The results of visualization using R
proliferation software showed that all 5 hub genes were highly expressed
GO:0090287 Regulation of cellular response 4 8.93e-04 in DCM (Figure 7).
to growth factor stimulus
GO:0002888 Positive regulation of myeloid 2 9.82e-04 3.5. ROC curve plotting
leukocyte mediated immunity In the ROC curve of GSE29819, all five hub genes had high
GO:0003299 Muscle hypertrophy in response 2 9.82e-04 value in diagnosing DCM relative to NF samples; OGN
to stress (AUC = 0.958) had the highest diagnostic value, followed
GO:0014887 Cardiac muscle adaptation 2 9.82e-04 by NPPA (AUC = 0.917), SFRP4 (AUC = 0.881), ASPN
GO:0014898 Cardiac muscle hypertrophy in 2 9.82e-04 (AUC = 0.863), and MME (AUC = 0.863), as shown
response to stress in Figure 8A. On the other hand, in the ROC curve
GO:0033687 Osteoblast proliferation 2 9.82e-04 of GSE57338, SFRP4 (AUC = 0.923) had the highest
GO:0043304 Regulation of mast cell 2 9.82e-04 diagnostic value, followed by ASPN (AUC = 0.914),
degranulation MME (AUC = 0.894), OGN (AUC = 0.891), and NPPA
(Contd...) (AUC = 0.747), as shown in Figure 8B.

Volume 1 Issue 1 (2022) 6 https://doi.org/10.36922/gtm.v1i1.104