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Global Translational Medicine

ORIGINAL RESEARCH ARTICLE
Hesperetin alleviates pulmonary injury in a blunt

chest trauma-induced pulmonary contusion
model in rats

2
Serkan Kaya 1 , İhsan Karaboğa * , Yasin Duran 3 , Elif Polat 4 ,
Fatin Rüştü Polat 5 , and Zeynep Fidanol Erboğa 4
1 Department of Thoracic Surgery, Tekirdağ Dr. İsmail Fehmi Cumalioğlu State Hospital, Tekirdag,
Türkiye
2 Department of Histology and Embryology, Kırklareli University, Faculty of Medicine, Kırklareli,
Türkiye
3 Department of Pathology Laboratory Techniques, Vocational School of Health Services, Istanbul
Rumeli University, Istanbul, Türkiye
4 Department of Histology and Embryology, Faculty of Medicine, Tekirdag Namık Kemal University,
Tekirdag, Türkiye
5 Department of General Surgery, Faculty of Medicine, Tekirdag Namık Kemal University, Tekirdag,
Türkiye

Abstract

Pulmonary contusion (PC), a condition that occurs frequently in severe thoracic
injuries, is a significant contributor to mortality in those under the age of 40.
Hesperetin, a natural flavonoid derivative of hesperidin, is a substance found in
*Corresponding author: various citrus fruits such as oranges and grapefruits and possesses a variety of
İhsan Karaboğa
(ihsankaraboga@klu.edu.tr) biological activities, including antiapoptotic, antioxidant, and anticancer effects.
In the current study, we investigated the effect of hesperetin on pulmonary
Citation: Kaya S, Karaboğa İ,
Duran Y, Polat E, Polat FR, Erboğa tissue structure, expression of some pro-inflammatory cytokines, and mediators
ZF. Hesperetin alleviates pulmonary in a PC model induced by blunt chest trauma. In this study, 18 adult male Wistar
injury in a blunt chest trauma- albino rats (8 – 10 weeks, 250 – 300 g) were used. The rats were divided into three
induced pulmonary contusion
model in rats. Global Transl Med. groups: control, PC, and PC + hesperetin. Hesperetin administration (100 mg/kg/
2024;3(2):2568. day oral) was completed for 7 days following induction of the model. The wet/dry
doi: 10.36922/gtm.2568 weight ratio of pulmonary tissue was determined. Tumor necrosis factor alpha
Received: December 29, 2023 (TNFα) and malondialdehyde (MDA) in lung tissues, serum interleukin (IL)-6, and
Accepted: May 14, 2024 IL-1β levels were determined using the enzyme-linked immunosorbent assays.
Published Online: June 11, 2024
Pulmonary tissue specimens were examined histologically using hematoxylin-
Copyright: © 2024 Author(s). eosin and Masson trichrome staining. Inducible nitric oxide synthase (iNOS)
This is an Open Access article activity was determined using immunohistochemical methods. Hesperetin
distributed under the terms of the
Creative Commons Attribution administration reduced TNFα and iNOS activity, serum IL-1β, IL-6, MDA, and
License, permitting distribution, wet/dry weight ratio in pulmonary tissue to improve pulmonary function. Our
and reproduction in any medium, results showed that administration of hesperetin prevents activation of local
provided the original work is
properly cited. inflammatory mediators, thereby obstructing the proinflammatory cytokine
cascade and tissue injury.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: Trauma; Tumor necrosis factor alpha; Malondialdehyde; Interleukin 6
affiliations.

Volume 3 Issue 2 (2024) 1 doi: 10.36922/gtm.2568