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Global Translational Medicine Angiotensinogen in liver steatosis

A

B

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D

Figure 1. Macrovesicular liver steatosis was present after 14 days of WD feeding in hepAGT +/+ mice. (A) Albumin-Cre 0/0; angiotensinogen floxed (f/f)
mice (hepAGT +/+) on an LDLR -/- background were fed WD for 0 (n = 4), 5 (n = 5), 14 (n = 5), or 42 (n = 5) days. (B) Representative images of H&E
and (C) Oil Red O staining in livers. Black arrowheads indicate large intrahepatic vacuoles. Scale bars = 50 µm. (D) Quantification of Oil Red O staining-
positive area. Numbers in the panel (D) are p-values calculated by Kruskal–Wallis test followed by Dunn’s method.
Abbreviations: H&E: Hematoxylin and eosin; LDLR: Low-density lipoprotein receptor; ND: Normal laboratory diet; WD: Western diet.

Red O-positive area between baseline and 5 days of WD (Figure 1C). Oil Red O-positive areas were increased
feeding (Figure 1C and D). At 14 days of WD feeding, a significantly at 14 and 42 days of WD feeding compared
subset of hepatocytes displayed large neutral lipid droplets to baseline (Figure 1D). These data suggest that 5, 14, and
surrounded by apparently displaced nuclei, indicative 42 days of WD feeding induce liver steatosis corresponding
of macrovesicular steatosis. At 42 days of WD feeding, to pre-pathological, initiation, and advanced phases,
macrovesicular steatosis was apparent in most hepatocytes respectively, in hypercholesterolemic mice.

Volume 4 Issue 2 (2025) 74 doi: 10.36922/gtm.6027