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Global Translational Medicine Angiotensinogen in liver steatosis

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Figure 4. Cell division-related genes elevated by WD feeding were suppressed in hepatocyte-specific AGT-deficient mice at 14 days of WD. (A) Flowchart of
two-armed bulk RNA sequencing. WD-induced liver transcriptomic alterations were analyzed in hepAGT +/+ mice after 5, 14, and 42 days of WD feeding
compared against 0 days of WD. Liver transcriptomic alterations due to hepatic AGT deficiency were analyzed after 14 days of WD feeding by comparing
hepAGT -/- mice against hepAGT +/+ mice. Both RNA sequencing data were integrated to identify target genes. (B) Venn diagram for overlapped
DEGs from combined transcriptomic analyses. Cluster 4 represents DEGs with upregulation consistent with steatotic progression in hepAGT +/+ mice.
hepAGT -/- versus +/+ represents DEGs altered in hepAGT -/- mice at 14 days of WD. (C) Log FC alteration in hepAGT -/- mice at 14 days of WD within
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overlapped DEGs. (D) Chord graph representing overlapped DEGs corresponding to the top five terms in gene ontology analysis. (E) Normalized read
count expression of six target DEGs in hepAGT +/+ and -/- mice after 14 days of WD feeding. False discovery rate-adjusted p-values are represented above
for each DEG.
Abbreviations: AGT: Angiotensinogen; DEG: Differentially expressed gene; FC: Fold change; hepAGT +/+: albumin-Cre 0/0 AGT floxed (f/f) low-density
lipoprotein-receptor-deficient background; hepAGT -/-: Albumin-Cre 1/0 AGT floxed (f/f) low-density lipoprotein-receptor-deficient background;
WD: Western diet.

of steatosis. The integration of the two sequencing data into the regulation of potentially causative genes with
highlighted 24 DEGs. Despite the small number of DEGs, less interference from genes altered as a consequence of
by focusing on the initiation phase, we gain insights steatosis.

Volume 4 Issue 2 (2025) 78 doi: 10.36922/gtm.6027