Page 320 - IJB-10-1

International

Journal of Bioprinting

RESEARCH ARTICLE
An in vitro model of meningioma constructed by

3D coaxial bioprinting

Peng Chen 1,2† , Yongan Jiang 1,2† , Hengyi Fan , Jianwei Chen , Raorao Yuan ,
1†
3
1
Fan Xu , Shiqi Cheng *, and Yan Zhang *
4
1
1
1 Department of Neurosurgery, The Second Affiliated Hospital of Nanchang University, Nanchang
330006, Jiangxi, China
2 School of Medicine, Nanchang University, Nanchang 330006, Jiangxi, China
3 Bio-intelligent Manufacturing and Living Matter Bioprinting Center, Research Institute of Tsinghua
University in Shenzhen, Tsinghua University, Shenzhen 518000, China
4 Department of Neurosurgery, Nanchang People’s Hospital, Nanchang County 330200, Jiangxi,
China

Abstract

Meningioma, a type of brain tumor, has a high incidence rate and requires a
comprehensive treatment approach due to its invasive nature and varying
malignancy. In vitro models for studying the molecular mechanisms of malignant
meningiomas and drug screening are urgently needed. However, two-dimensional
(2D) culture of meningioma cells has limitations and challenges. Three-dimensional
† These authors contributed equally (3D) printing technology can provide a more realistic in vitro research platform for
to this work. tumor research. In this study, 3D coaxial bioprinting was used to fabricate an in vitro
*Corresponding authors: 3D model of meningioma that faithfully recapitulates the biological characteristics
Shiqi Cheng and microenvironment of this malignancy. The bioprinted construct features a fibrous
(doctorchengshiqi@outlook.com) core-shell structure that allows cell clustering and fusion into cell fibers, ultimately
Yan Zhang forming a complex 3D structure resembling meningioma. Our findings suggest that
(doctorzhangyan@outlook.com)
the 3D model of meningioma generated by coaxial bioprinting closely resembles
Citation: Chen P, Jiang Y, Fan H, the in vivo morphology and growth pattern. Compared with 2D culture, 3D culture
et al. An in vitro model of
meningioma constructed by 3D conditions better simulated the tumor microenvironment and exhibited higher
coaxial bioprinting. Int J Bioprint. invasiveness and tumorigenicity. Comparative analysis of biomarker expression
2024;10(1):1342. further demonstrated that 3D culture provides a more accurate reflection of the
doi: 10.36922/ijb.1342
biological characteristics of tumors. Our research affirms that microtissue models
Received: July 20, 2023 produced by 3D coaxial bioprinting can replicate the in vivo environment of cancer
Accepted: August 7, 2023
Published Online: September 1, cells, producing survival conditions that are nearly realistic and more conducive to
2023 the restoration of cancer cell traits in vitro.
Copyright: © 2023 Author(s).
This is an Open Access article Keywords: Bioprinting; Coaxial; Meningioma; Self-assembling; In vitro model
distributed under the terms of the
Creative Commons Attribution
License, permitting distribution,
and reproduction in any medium,
provided the original work is
properly cited. 1. Introduction
Publisher’s Note: AccScience Meningioma, a type of tumor originating from the meninges, accounts for approximately
Publishing remains neutral with 15% to 20% of intracranial tumors and has an incidence of 5–10 cases per 1 million
regard to jurisdictional claims in 1-2
published maps and institutional population. Meningiomas can be classified into benign and malignant, with malignant
3
affiliations. meningiomas accounting for approximately 20%. Due to their faster growth rate,

Volume 10 Issue 1 (2024) 312 https://doi.org/10.36922/ijb.1342