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Polyelectrolyte gelatin-chitosan hydrogel optimized for 3D bioprinting in skin tissue engineering























                    Figure 8. Live-dead staining on Day 4. (Left) chitosan hydrogel, (Right) 5% PGC hydrogel (scale bar: 100 μm).

            mature to form a functional tissue. As such, 3D bio-
            printing  serves as an  attractive platform to  facilitate   Conflict of Interest and Funding
            cellular and matrix deposition in a spatially-controlled   No conflict of interest was reported by the authors.
            3D  matrix. Chitosan is a promising polymer used in
            wound  healing applications due  to  its antimicrobial   Acknowledgments
            and hemostasis properties. In this work, modification   The first author  would like to thank the scholarship
            to the chitosan was carried out via the addition of ge-  sponsorship by A*STAR Graduate Academy.
            latin to form printable polyelectrolyte gelatin-chitosan
            (PGC) hydrogels. We have optimized PGC hydrogels   References
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            exhibited a sufficiently high viscosity that is suitable   1.   Lanza R, Langer R and Vacanti J P, 2011, Principles of
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            60                          International Journal of Bioprinting (2016)–Volume 2, Issue 1
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