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Kenichi Arai, Yoshinari Tsukamoto, Hirotoshi Yoshida, et al.

chanism through the enzymatic reaction of several http://dx.doi.org/10.1124/dmd.111.041137
biomaterials bearing Ph moieties and HRP in order to 8. Jiankang H, Dichen L, Yaxiong L, et al., 2009, Prepara-
fabricate 3D gel structures with cell-adhesive properties. tion of chitosan-gelatin hybrid scaffolds with well-orga-
Alg-Ph and Alg-Ph/Gelatin-Ph gels were examined. It nized microstructures for hepatic tissue engineering.
was confirmed that the fibroblasts were viable for 7 Acta Biomaterialia, vol.5(1): 453–461.
days and extended well especially in Alg-Ph/Gelatin- http://dx.doi.org/10.1016/j.actbio.2008.07.002
Ph hydrogels. Finally, using Alg-Ph/Gelatin-Ph as ink 9. Tsuda Y, Kikuchi A, Yamato M, et al., 2006, Heterotypic
materials, the 3D gel structure was successfully fabri- cell interactions on a dually patterned surface. Bio-
cated. We conclude that Alg-Ph/Gelatin- Ph is a good chemical and Biophysical Research Communications,
cell-adhesive bioink material for the fabrication of 3D vol.348(3): 937–944.
cell-adhesive gel structures via 3D- bioprinter. http://dx.doi.org/10.1016/j.bbrc.2006.07.138
10. Yamada M, Utoh R, Ohashi K, et al., 2012, Controlled
Conflict of Interest and Funding formation of heterotypic hepatic micro-organoids in ani-
sotropic hydrogel microfibers for long-term preservation
No conflict of interest was reported by all authors. This of liver-specific functions. Biomaterials, vol.33(33):
work was supported by Grants-in-Aid for Scientific Re- 8304–8315.
search: No.26106713, No.26670410 and 15550217 http://dx.doi.org/10.1016/j.biomaterials.2012.07.068
from the Japan Society for the Promotion of Science. 11. Malda J, Visser J, Melchels F P, et al., 2013, 25th anni-
versary article: engineering hydrogels for biofabrication.
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