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The development of cell-adhesive hydrogel for 3D printing

Figure 6. Observation of cell morphology in hydrogels. Photomicrographs of fibroblasts in Alg-gel (A), Alg-Ph gel (B), and
Alg-Ph/Gelatin-Ph gel (C). Fibroblasts were stained with Hoechst 33342 and Alexa Fluor 488 phalloidin. Phase contrast images of
fibroblasts in Alg-gel (D), Alg-Ph gel (E), and Alg-Ph/Gelatin-Ph gel (F). The scale bar is 100 µm.

Figure 7. Fabrication of 3D gel sheet structure by using a 3D-bioprinter. Bitmap image of mesh structure (A) and 3D computer mod-
el (B). The fabricated 3D gel sheet strutrure of Alg-Ph/Gelatin-Ph by using 3D-bioprinter (C). The fabricated gel structure was ob-
served by using a fluorescence microscope (D).

including living cells was fabricated and cultured at hand, the fibroblasts were confirmed to extend in the 3D
different time periods, i.e., 1 day or 7 days (Figures 8–9). gel sheet after 7 days of cultivation (Figures 9D–9F).
After cultivation, the F-actin of fibroblasts in the 3D gel
sheet structure was stained and observed. It was found 4. Discussion
that fibroblast morphology was maintained in the fa- There is an increasing demand in tissue engineering
bricated gels at day 1 (Figures 8D–8F). On the other and regenerative medicine research to construct 3D

158 International Journal of Bioprinting (2016)–Volume 2, Issue 2