Page 130 - IJB-10-2

International Journal of Bioprinting 3D bioprinting for corneal regeneration

Table 2. (Continued)
Epithelium and Animal model: Collagen-copolymer Robust, suturable scaffold; 91,115
anterior stroma pig refractive index: 1.343 (human tier film: 1.336);
optically clear;
good glucose permeability;
intraocular pressure after surgery: 10–16 Hgmm (10–13 Hgmm
before surgery)
Epithelium and Animal model: Cross-linked recom- Type III collagen gels were more transparent (87–92%) than human 91,116
anterior stroma pig binant human Type I cornea (87%) depending on cross-linker concentration;
and Type III collagen refractive index (1.3451 to 1.3552) lower than human cornea (1.373
to 1.380);
fully biocompatible in vitro; promoted regeneration of cells and
nerves in vivo
Stroma In vitro study Magnetically oriented Oriented collagen sheets prepared 91,117
collagen/proteoglycan HDFs colonized the gel successfully; collagen concentration (2 mg/
hydrogels mL) was very low compared to native cornea;
concentrated scaffold was not transparent but was improved by
proteoglycans;
cells could align to the orientation of collagen gel
Magnetically oriented Non-cytotoxic; 91,118
Rat tail type I collagen cells could penetrate and remodel scaffold;
cells in gel had aligned multilayered structures;
cells produce collagen in the matrix but degrade the original
scaffold
Stroma In vitro study Bovine collagen film Scaffold size showed a 10% decrease during 4 weeks of culture; 91,119
fibroblast cells flattened after 1 week of culturing on film and
formed multilayer;
cells penetrated the film itself;
surface roughness: 0.987 nm (cornea: 1.197 nm)
Stroma In vitro and in Gelatin hydrogels 4-week follow-up; 91,120
vivo study in vivo, cells expressed more vimentin than in vitro;
precursor in gel had better results on collagen and vimentin expres-
sion;
gelatin in gel promotes only ECM production;
ECM and vimentin production arise from host species, not from
transplanted cells
Stroma In vitro study Surface patterned Silk film was fully transparent; 73,91
silk foil cells aligned to the circular pattern of silk film;
proliferation was lower than on TCP;
cell density was higher on patterned silk than on flat silk film;
high expression of type V collagen and vimentin;
films stackable to create 3D structures
Stroma In vitro study Silk foil with porous RGD functionalized silk films; 91,121,122
and patterned surface cells do not grow to confluent without RGD;
cell morphology was more elongated on the patterned surface;
cells proliferate more on patterned/porous silk film with RGD
RGD content results in stronger expression of ECM;
stacked silk films had good transparency
Endothelium In vitro and in Decellularized amni- Cells formed a confluent monolayer on dAC; 91,123
vivo study on cat otic membrane dAC and cell-loaded dAC were transplanted into cats;
model inflammation in the cell-loaded group decreased more;
cell-loaded dAC group had less or no opaque cornea;
cell morphology on dAC was similar to normal culture
(Continued)

Volume 10 Issue 2 (2024) 122 doi: 10.36922/ijb.1669