Page 181 - IJB-10-2

International Journal of Bioprinting Coronavirus-infected bioprinted intestine

Figure 4. Synergistic strategies and advantages of merging intestinal organoids and organ-on-chip platforms for HCoV infection investigations. (A) The
fusion of organoids and organ-on-chip technology gives rise to organoid-on-chip configurations, enhancing physiological fidelity. Within microfluidic
systems under continuous perfusion, a diverse array of organoids can be mono-/co-cultured, while organ-on-chip setups enable the creation of vascularized
organoid models. (B) Within the context of intestinal organoid-on-chip, cells derived from dissociated intestinal organoids serve as a pivotal source,
faithfully emulating the human gut environment. This integrated model facilitates streamlined cell differentiation, heightened expression of virus-binding
receptors, and extended cellular viability through continuous perfusion. This illustration was created with Biorender.com.

fibroblast-laden crypt-villus structures, faithfully yields functional gut mucosa tissues compatible with
replicating the intestinal tissue’s 3D complexity. This conventional testing techniques. These biofabricated gut
process, based on digital light projection stereolithography mucosa tissues can serve as advanced in vitro models
(DLP-SLA), demonstrated excellent cell viability, for studying human coronaviruses, providing valuable
accurate spatial resolution, and high printing throughput, insights into viral pathogenesis and host responses. In
82
representing a robust biofabrication approach that another groundbreaking study, a customized DLP-SLA

Volume 10 Issue 2 (2024) 173 doi: 10.36922/ijb.1704