Page 236 - IJB-10-2

International

Journal of Bioprinting

RESEARCH ARTICLE
Building a degradable scaffold with 3D printing

using Masquelet technique to promote
osteoblast differentiation and angiogenesis in

chronic tibial osteomyelitis with bone defects

Fan Liu, Chaohan Wu, Xinhui Wang, Rongkang Guo, Tianhua Dong, and Tao Zhang*
Department of Emergency Trauma Center, The Third Hospital of Hebei Medical University,
Shijiazhuang, Hebei, China

Abstract

The aim of this study was to investigate the use of three-dimensional (3D) printing
technology to create a biodegradable scaffold loaded with WNT5A protein and
assess its impact on chronic tibial osteomyelitis with bone defects (CTO&BD),
focusing on osteoblast differentiation and angiogenesis. We extracted RNA from
peripheral blood of healthy individuals and CTO&BD patients for sequencing,
followed by differential expression and functional enrichment analysis. Network
*Corresponding author: analysis was performed to identify core genes associated with CTO&BD and
Tao Zhang construct a protein–protein interaction network. Using Masquelet technique, we
(zt50500@hebmu.edu.cn)
fabricated a 3D-printed biodegradable scaffold (G40T60@WNT5A) and conducted
Citation: Liu F, Wu C, Wang X, various experiments, including rheological testing, printability evaluation, Fourier-
Guo R, Dong T, Zhang T. Building
a degradable scaffold with 3D transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy
printing using Masquelet technique analysis, as well as mechanical and degradation performance assessments. In in vivo
to promote osteoblast differentiation experiments, we observed the formation of induced membranes in a CTO&BD rat
and angiogenesis in chronic tibial
osteomyelitis with bone defects. model implanted with the scaffold. In vitro experiments involved the assessment
Int J Bioprint. 2024;10(2):1461. of scaffold toxicity on rat bone marrow mesenchymal stem cells and umbilical
doi: 10.36922/ijb.1461 vein endothelial cells, as well as the influence on osteoblast differentiation and
Received: August 7, 2023 angiogenesis. Molecular biology techniques were used to analyze gene and protein
Accepted: October 10, 2023 expression levels. We discovered for the first time that WNT5A may play a crucial role
Published Online: January 9, 2024 in CTO&BD. The biodegradable scaffold prepared by 3D printing (G40T60@WNT5A)
Copyright: © 2024 Author(s). exhibited excellent biocompatibility in vitro. This scaffold significantly promoted the
This is an Open Access article formation of induced membranes in CTO&BD rats and further enhanced osteoblast
distributed under the terms of the
Creative Commons Attribution differentiation and angiogenesis. In conclusion, this study utilized innovative
License, permitting distribution, 3D printing technology to fabricate the G40T60@WNT5A scaffold, confirming
and reproduction in any medium, its potential application in the treatment of CTO&BD, particularly in promoting
provided the original work is
properly cited. osteoblast differentiation and angiogenesis. This research provides new methods
and theoretical support for the treatment of bone defects.
Publisher’s Note: AccScience
Publishing remains neutral with
regard to jurisdictional claims in
published maps and institutional Keywords: 3D printing; WNT5A; Chronic tibial osteomyelitis; Bone defect;
affiliations. Osteogenic differentiation; Scaffold transplantation

Volume 10 Issue 2 (2024) 228 doi: 10.36922/ijb.1461