Page 287 - IJB-10-2

International

Journal of Bioprinting

RESEARCH ARTICLE
Decellularized porcine kidney-incorporated

hydrogels for cell-laden bioprinting of renal
cell carcinoma model

Miaoben Wu 1,2† , Hangyu Zhou , Jingying Hu , Zonghuan Wang ,
2
2†
3
2
1
1
Yongqi Xu , Yibing Wu , Yang Xiang , Jun Yin , Peng Wei , Kailei
1
4
Xu 5,6* , and Tiantian Ren 7*
1 Department of Plastic and reconstructive surgery, The First Affiliated Hospital of Ningbo University,
Ningbo, Zhejiang, China
2 Health Science Center, Ningbo University, Ningbo, Zhejiang, China
3 Department of Medical Research Center, the First Affiliated Hospital of Ningbo University, Ningbo,
Zhejiang, China
4 The State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical
Engineering, Zhejiang University, Hangzhou, Zhejiang, China
5
Center for Medical and Engineering Innovation, Central Laboratory, The First Affiliated Hospital of
Ningbo University, Ningbo, Zhejiang, China
6 Key Laboratory of Precision Medicine for Atherosclerotic Diseases of Zhejiang Province, Ningbo,
Zhejiang, China
7 Department of Trauma Surgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China
(This article belongs to the Special Issue: 3D Bioprinting for Tumor Modeling)
† These authors contributed equally
to this work. Abstract
*Corresponding authors:
Tiantian Ren More than 90% of kidney cancers are attributed to renal cell carcinoma (RCC), which is
(fyyrentiantian@nbu.edu.cn) however highly resistant to traditional chemotherapy. The challenges met in treating RCC
Kailei Xu signify an imperative to accelerate the development of new and effective drugs. Preclinical
(xukailei@zju.edu.cn) testing has served as a foundation for evaluating potential effectiveness of new drugs, but
Citation: Wu M, Zhou H, Hu J, this endeavor is deeply restricted by the current generation of in vitro two-dimensional
et al. Decellularized porcine culture models, which cannot accurately mimic the tumor microenvironment (TME).
kidney-incorporated hydrogels for
cell-laden bioprinting of renal cell Therefore, new in vitro three-dimensional (3D) cell culture models that can better mimic
carcinoma model. Int J Bioprint. the components and architecture of TME have been developed for preclinical testing, but
2024;10(2):1413. only a few existing 3D cell culture models can simulate the TME of RCC, representing a
doi: 10.36922/ijb.1413
limitative obstacle impeding the development of novel drugs for RCC. In this study, we
Received: July 29, 2023 prepared a bioink by mixing porcine kidney decellularized extracellular matrix (dECM)
Accepted: October 18, 2023
Published Online: January 12, 2024 powders with gelatin methacryloyl (GelMA) to bioprint an in vitro 3D cell culture model
for RCC. We found that GelMA stability, mechanical properties, and printability were
Copyright: © 2024 Author(s). all significantly improved following the addition of the dECM powder. Moreover, cell
This is an Open Access article
distributed under the terms of the cultures using ACHN cells suggested that kidney dECM powders significantly improved
Creative Commons Attribution the cellular proliferation and metastasis via upregulation of markers related to epithelial–
License, permitting distribution, mesenchymal transition, along with activation of several cancer progression-related
and reproduction in any medium,
provided the original work is signaling pathways. More importantly, ACHN cells also demonstrated higher resistance
properly cited. to sunitinib under the stimulation of kidney dECM, indicating that GelMA-kidney dECM
Publisher’s Note: AccScience hydrogels may be an appropriate preclinical model to be used for building an in vitro RCC
Publishing remains neutral with platform for drug screening and development.
regard to jurisdictional claims in
published maps and institutional Keywords: Tumor microenvironment; Gelatin methacrylate; 3D culture; Tumor
affiliations.
model; Drug screening

Volume 10 Issue 2 (2024) 279 doi: 10.36922/ijb.1413