Page 339 - IJB-10-2

International

Journal of Bioprinting

RESEARCH ARTICLE
3D-bioprinted cell-laden blood vessel with

dual drug delivery nanoparticles for advancing
vascular regeneration

Eun Ji Lee 1,2† , Jaewoo Choi 1,2† , Hye Ji Lim , Deokhyeon Yoon , Dong Myoung
1,2
1,2
Lee , Donggu Kang , Jeong-Seok Lee , Hojun Jeon , Kyeong Hyeon Lee ,
3
1,2
3
4
3
Yong-Il Shin , Sang-Cheol Han , Woong Bi Jang *, and Sang-Mo Kwon *
6
4,5
1,2
1,2
1 Laboratory for Vascular Medicine and Stem Cell Biology, Department of Physiology, Medical
Research Institute, School of Medicine, Pusan National University, Yangsan, Republic of Korea
2 Convergence Stem Cell Research Center, Pusan National University, Yangsan, Republic of Korea
3 Research Institute of Additive Manufacturing and Regenerative Medicine, Baobab Healthcare Inc.,
55 Hanyangdaehak-Ro, Ansan, Gyeonggi-do, South Korea
4 Science of Convergence, School of Medicine, Pusan National University, Yangsan, Republic of
Korea
5 Department of Rehabilitation Medicine, Pusan National University Yangsan Hospital, Yangsan,
Republic of Korea
6 CEN Co., Ltd., Nano-Convergence Center, 761 Muan-ro, Miryang, Republic of Korea
(This article belongs to the Special Issue: Nano-enabled 3D Bioprinting for Various Tissue Engineering)
Abstract
† These authors contributed equally
to this work. Vascular regeneration plays a critical role in the treatment of cardiovascular
*Corresponding authors: diseases and in tissue engineering applications. In this study, we fabricated and
Woong Bi Jang characterized statin/curcumin-loaded nanoparticles for potential applications in
(jangwoongbi@naver.com) vascular regeneration. The nanoparticles exhibited consistent spherical shape and
Sang-Mo Kwon sizes, indicating reproducibility and stability of the fabrication process. The sustained
(smkwon323@pusan.ac.kr)
release of the loaded drugs from the nanoparticles indicated their suitability for
Citation: Lee EJ, Choi J, Lim HJ, controlled and prolonged drug delivery. Biocompatibility assessments revealed that
et al. 3D-bioprinted cell-laden blood the nanoparticles were nontoxic even at high concentrations and over extended
vessel with dual drug delivery
nanoparticles for advancing periods. Moreover, the incorporation of statin within the nanoparticles enhanced
vascular regeneration. Int J Bioprint. the proliferative capacity and functional abilities of endothelial progenitor cells,
2024;10(2):1857. thereby promoting angiogenesis and vascular repair. Co-administration of curcumin
doi: 10.36922/ijb.1857
with statin further augmented the therapeutic effects by reducing intracellular
Received: September 18, 2023 reactive oxygen species levels and providing antioxidant protection against
Accepted: November 17, 2023
Published Online: January 12, 2024 oxidative stress. Furthermore, we successfully integrated these nanoparticles
into artificial blood vessels (ABVs) using three-dimensional printing technology,
Copyright: © 2024 Author(s).
This is an Open Access article creating customizable constructs capable of supporting vascular regeneration. The
distributed under the terms of the viability and proliferative capacity of cells within the ABVs were preserved, which
Creative Commons Attribution has potential for targeted drug delivery and localized therapy. In in vivo models
License, permitting distribution,
and reproduction in any medium, of hindlimb ischemia, transplantation of nanoparticle-loaded ABVs resulted in
provided the original work is significant improvements in terms of recovery speed and blood flow. Histological
properly cited. analysis confirmed the enhanced expression of vascular-related markers, indicating
Publisher’s Note: AccScience improved angiogenesis. Collectively, our findings demonstrate the potential of
Publishing remains neutral with statin/curcumin-loaded nanoparticles as a promising approach for vascular tissue
regard to jurisdictional claims in engineering and regenerative medicine. These nanoparticles offer controlled drug
published maps and institutional
affiliations. delivery, biocompatibility, and enhanced regenerative properties, suggesting that

Volume 10 Issue 2 (2024) 331 doi: 10.36922/ijb.1857